Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis

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dc.contributor.authordos Santos, Jomel Francisco
dc.contributor.authordo Rego, Rafael Otaviano
dc.contributor.authorBastos Afonso, José Augusto
dc.contributor.authorFagliari, José Jurandir [UNESP]
dc.contributor.authorSilva, Paulo Cesar [UNESP]
dc.contributor.authorSoares, Pierre Castro
dc.contributor.authorde Mendonça, Carla Lopes
dc.contributor.institutionCMZL
dc.contributor.institutionUniversidade Federal do Espírito Santo (UFES)
dc.contributor.institutionUFRPE
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-06-25T11:08:37Z
dc.date.available2021-06-25T11:08:37Z
dc.date.issued2021-01-01
dc.description.abstractThis study aimed to evaluate the hematologic response and the serum and peritoneal fluid (PF) proteinogram of cattle affected by digestive diseases. Twenty-seven animals were distributed in two groups: GI (intestinal diseases) and GII (traumatic reticuloperitonitis, TRP). The animals were previously submitted to a physical exam. Subsequently, blood samples were collected to perform the complete blood count, determine the plasma protein and fibrinogen, and obtain the serum for proteinogram in polyacrylamide gel (SDS-PAGE). Simultaneously, PF was collected to perform physical and chemical evaluation and the electrophoretic profile (SDS-PAGE). ANOVA at the 5% probability level was used to compare the groups. The animals showed signs of apathy, dehydration, and gastrointestinal hypomotility in both groups. However, GI animals showed more significant clinical changes. The blood count of both groups (P > 0.05) showed leukocytosis due to neutrophilia and a regenerative left shift with hyperfibrinogenemia. The proteinogram of both body fluids allowed the identification of proteins albumin (ALB), transferrin (TRF), ceruloplasmin, haptoglobin, α1-acid glycoprotein (α1-AGP), MW 23000 Da, α1-antitrypsin, IgA, and IgG. The [PT] PF/[PT] blood serum ratio of each of the identified proteins increased, showing statistical differences between groups (P < 0.05) regarding PT, ALB, TRF, α1-AGP, and IgG values, with GI animals showing the highest ratio. Intestinal diseases and TRP triggered a systemic and local response characterized by clinical, hematological, and serum and PF proteinogram alterations. The proteins α1-GPA, haptoglobin, and TRF measured in PF were good inflammation biomarkers and useful as an auxiliary tool for the diagnosis and prognosis of digestive diseases in cattle.en
dc.description.affiliationInstituto Federal de Educação Ciência e Tecnologia do Amazonas Campus Manaus Zona Leste IFAM CMZL
dc.description.affiliationUniversidade Federal do Espírito Santo Campus Alegre UFES
dc.description.affiliationClínica de Bovinos de Garanhuns Campus Garanhuns Universidade Federal Rural de Pernambuco CBG UFRPE
dc.description.affiliationFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Júlio de Mesquita Filho UNESP Campus de Jaboticabal
dc.description.affiliationFaculdade de Ciências Agrárias e Veterinárias UNESP Campus de Jaboticabal
dc.description.affiliationDepartamento de Medicina Veterinária DMV UFRPE
dc.description.affiliationUnespFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Júlio de Mesquita Filho UNESP Campus de Jaboticabal
dc.description.affiliationUnespFaculdade de Ciências Agrárias e Veterinárias UNESP Campus de Jaboticabal
dc.format.extent209-228
dc.identifierhttp://dx.doi.org/10.5433/1679-0359.2021v42n1p209
dc.identifier.citationSemina:Ciencias Agrarias, v. 42, n. 1, p. 209-228, 2021.
dc.identifier.doi10.5433/1679-0359.2021v42n1p209
dc.identifier.issn1679-0359
dc.identifier.issn1676-546X
dc.identifier.scopus2-s2.0-85097490613
dc.identifier.urihttp://hdl.handle.net/11449/208228
dc.language.isoeng
dc.relation.ispartofSemina:Ciencias Agrarias
dc.sourceScopus
dc.subjectAbdominal fluid
dc.subjectAcute-phase proteins
dc.subjectCows
dc.subjectDigestive diseases
dc.subjectSDS-PAGE
dc.titleHematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitisen
dc.titleResposta hematológica e proteinograma sérico e do líquido peritoneal de bovinos acometidos com enfermidades intestinais e reticuloperitonite traumáticapt
dc.typeArtigo
unesp.departmentClínica e Cirurgia Veterinária - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias