MetaPPase by in silico analyses predicting the novel gene operate as H+ pumps.

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dc.contributor.authorRodrigues, Gisele Regina
dc.contributor.authorVal-Moraes, Silvana Pompeia
dc.contributor.authorLemos, Eliana Gertrudes Macedo
dc.contributor.authorPizauro Júnior, João Martins [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-05-15T13:30:31Z
dc.date.available2015-05-15T13:30:31Z
dc.date.issued2014
dc.description.abstractThe lastyears declined the discovery of compounds to use in industrial and naturaldiversity has been the best supplier for novel genes, enzymes and compounds inhigh demand by the biotechnology industry. We know immense diversity of microorganisms,yet most remains unexplored. For these reason we use the metagenômica approach toinvestigate the potential of uncultured microorganisms. With this purpose weused the metagenomic library of from Eucalyptus spp. arboretum (EAA), wedid screening to found positive clone and them was submitted to the process of shotgun,the data obtained was submitted a bioinformatics analyses. Our results showsthe hypothesis of high unexplored microbial diversity of soil are able to foundnovel genes and metagenomic approach is and allowed to isolate novel genes and insilico analyses are essential part to identify a novel Inorganicpyrophosphatase (PPase) prediction indicated the novel gene operate as H+ pumps. Thissuggests that a special feature, our work in situ will be cloning thegene expression vector for subsequent kinetic characterization and crystallization.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Tecnologia, Faculadade de Ciências Agrárias e Veterinárias Jaboticabal, Jaboticabal, Via deAcesso Prof Paulo Donato Castellani s/n, Rural, CEP 14884-900, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Tecnologia, Faculadade de Ciências Agrárias e Veterinárias Jaboticabal, Jaboticabal, Via deAcesso Prof Paulo Donato Castellani s/n, Rural, CEP 14884-900, SP, Brasil
dc.format.extent300-305
dc.identifierhttps://sites.google.com/site/topmostphotonjournals/home/the-journal-of-ecology
dc.identifier.citationThe Journal of Ecology, v. 1, n. 1, p. 300-305, 2014.
dc.identifier.issn6853-3275
dc.identifier.lattes3958124498479090
dc.identifier.lattes3902020936480943
dc.identifier.lattes4462378046178590
dc.identifier.orcid0000-0002-1119-7748
dc.identifier.urihttp://hdl.handle.net/11449/123620
dc.language.isoeng
dc.relation.ispartofThe Journal of Ecology
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectSoil diversityen
dc.subjectMembrane proteinen
dc.subjectIon transporten
dc.titleMetaPPase by in silico analyses predicting the novel gene operate as H+ pumps.en
dc.typeArtigo
unesp.author.lattes3958124498479090
unesp.author.lattes3902020936480943[3]
unesp.author.lattes4462378046178590
unesp.author.orcid0000-0002-1119-7748[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentTecnologia - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias