Effect of water storage and heat treatment on the cytotoxicity of soft liners

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dc.contributor.authorTay, Lidia Yileng
dc.contributor.authorHerrera, Daniel Rodrigo
dc.contributor.authorCosta Quishida, Cristiane Campos [UNESP]
dc.contributor.authorCarlos, Iracilda Zeppone [UNESP]
dc.contributor.authorJorge, Janaina Habib [UNESP]
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:46:32Z
dc.date.available2014-05-20T13:46:32Z
dc.date.issued2012-06-01
dc.description.abstractObjective: To evaluate the effect of water storage time on the cytotoxicity of soft liners.Methods: Sample discs of soft liners Dentusoft, Dentuflex, Trusoft, Ufi-Gel-P and denture base acrylic resin Lucitone-550 were prepared and divided into four groups: GN: No treatment, G24: Stored in water at 37 degrees C for 24 h; G48: Stored in water at 37 degrees C for 48 h, GHW: Immersed in water at 55 degrees C for 10 min. To analyse the cytotoxic effect, three samples of each group were placed in tubes with Dubelcco's Modified Eagle Mediums and incubated at 37 degrees C for 24 h. During this period, the toxic substances were leached to the culture medium. The cytotoxicity was analysed quantitatively by the incorporation of radioactivity H-3-thymidine checking the number of viable cells (synthesis of DNA). The data were statistically analysed using two-way ANOVA and Tukey's honestly significant difference tests (alpha = 0.05).Results: Treatments did not reduce the cytotoxicity effect of the soft liners (p > 0.05). It was found that Ufi-Gel-P had a non-cytotoxic effect, Trusoft had a slightly cytotoxic effect, Dentuflex had a moderated cytotoxic effect, Dentusoft alternated between slightly and non-cytotoxic effect, and Lucitone-550 had non-cytotoxic effect when stored in water for 48 h.Conclusion: The effect of water storage and the heat treatment did not reduce the cytotoxicity of the soft liners.en
dc.description.affiliationUniv Estadual Campinas, Sch Dent Piracicaba, São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, Dept Dent Mat & Prosthodont, Araraquara Dent Sch, São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, Araraquara Pharmaceut Sch, Dept Clin Anal, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Dent Mat & Prosthodont, Araraquara Dent Sch, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Araraquara Pharmaceut Sch, Dept Clin Anal, São Paulo, Brazil
dc.format.extentE275-E280
dc.identifierhttp://dx.doi.org/10.1111/j.1741-2358.2011.00463.x
dc.identifier.citationGerodontology. Hoboken: Wiley-blackwell, v. 29, n. 2, p. E275-E280, 2012.
dc.identifier.doi10.1111/j.1741-2358.2011.00463.x
dc.identifier.issn0734-0664
dc.identifier.urihttp://hdl.handle.net/11449/16482
dc.identifier.wosWOS:000308547000041
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofGerodontology
dc.relation.ispartofjcr1.439
dc.relation.ispartofsjr0,627
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectacrylic resinen
dc.subjectbiocompatibilityen
dc.subjectcell viabilityen
dc.subjectpolymethyl methacrylateen
dc.titleEffect of water storage and heat treatment on the cytotoxicity of soft linersen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
unesp.author.orcid0000-0002-8952-1202[2]
unesp.author.orcid0000-0002-0084-3468[4]
unesp.author.orcid0000-0002-9135-3455[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt
unesp.departmentAnálises Clínicas - FCFpt

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Artigos - Materiais Odontológicos e Prótese - FOAR
Araraquara - FCF - Faculdade de Ciências Farmacêuticas