Effect of water storage and heat treatment on the cytotoxicity of soft liners
dc.contributor.author | Tay, Lidia Yileng | |
dc.contributor.author | Herrera, Daniel Rodrigo | |
dc.contributor.author | Costa Quishida, Cristiane Campos [UNESP] | |
dc.contributor.author | Carlos, Iracilda Zeppone [UNESP] | |
dc.contributor.author | Jorge, Janaina Habib [UNESP] | |
dc.contributor.institution | Universidade Estadual de Campinas (UNICAMP) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-05-20T13:46:32Z | |
dc.date.available | 2014-05-20T13:46:32Z | |
dc.date.issued | 2012-06-01 | |
dc.description.abstract | Objective: To evaluate the effect of water storage time on the cytotoxicity of soft liners.Methods: Sample discs of soft liners Dentusoft, Dentuflex, Trusoft, Ufi-Gel-P and denture base acrylic resin Lucitone-550 were prepared and divided into four groups: GN: No treatment, G24: Stored in water at 37 degrees C for 24 h; G48: Stored in water at 37 degrees C for 48 h, GHW: Immersed in water at 55 degrees C for 10 min. To analyse the cytotoxic effect, three samples of each group were placed in tubes with Dubelcco's Modified Eagle Mediums and incubated at 37 degrees C for 24 h. During this period, the toxic substances were leached to the culture medium. The cytotoxicity was analysed quantitatively by the incorporation of radioactivity H-3-thymidine checking the number of viable cells (synthesis of DNA). The data were statistically analysed using two-way ANOVA and Tukey's honestly significant difference tests (alpha = 0.05).Results: Treatments did not reduce the cytotoxicity effect of the soft liners (p > 0.05). It was found that Ufi-Gel-P had a non-cytotoxic effect, Trusoft had a slightly cytotoxic effect, Dentuflex had a moderated cytotoxic effect, Dentusoft alternated between slightly and non-cytotoxic effect, and Lucitone-550 had non-cytotoxic effect when stored in water for 48 h.Conclusion: The effect of water storage and the heat treatment did not reduce the cytotoxicity of the soft liners. | en |
dc.description.affiliation | Univ Estadual Campinas, Sch Dent Piracicaba, São Paulo, Brazil | |
dc.description.affiliation | São Paulo State Univ, Dept Dent Mat & Prosthodont, Araraquara Dent Sch, São Paulo, Brazil | |
dc.description.affiliation | São Paulo State Univ, Araraquara Pharmaceut Sch, Dept Clin Anal, São Paulo, Brazil | |
dc.description.affiliationUnesp | São Paulo State Univ, Dept Dent Mat & Prosthodont, Araraquara Dent Sch, São Paulo, Brazil | |
dc.description.affiliationUnesp | São Paulo State Univ, Araraquara Pharmaceut Sch, Dept Clin Anal, São Paulo, Brazil | |
dc.format.extent | E275-E280 | |
dc.identifier | http://dx.doi.org/10.1111/j.1741-2358.2011.00463.x | |
dc.identifier.citation | Gerodontology. Hoboken: Wiley-blackwell, v. 29, n. 2, p. E275-E280, 2012. | |
dc.identifier.doi | 10.1111/j.1741-2358.2011.00463.x | |
dc.identifier.issn | 0734-0664 | |
dc.identifier.uri | http://hdl.handle.net/11449/16482 | |
dc.identifier.wos | WOS:000308547000041 | |
dc.language.iso | eng | |
dc.publisher | Wiley-Blackwell | |
dc.relation.ispartof | Gerodontology | |
dc.relation.ispartofjcr | 1.439 | |
dc.relation.ispartofsjr | 0,627 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | acrylic resin | en |
dc.subject | biocompatibility | en |
dc.subject | cell viability | en |
dc.subject | polymethyl methacrylate | en |
dc.title | Effect of water storage and heat treatment on the cytotoxicity of soft liners | en |
dc.type | Artigo | |
dcterms.license | http://olabout.wiley.com/WileyCDA/Section/id-406071.html | |
dcterms.rightsHolder | Wiley-blackwell | |
unesp.author.orcid | 0000-0002-8952-1202[2] | |
unesp.author.orcid | 0000-0002-0084-3468[4] | |
unesp.author.orcid | 0000-0002-9135-3455[5] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquara | pt |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquara | pt |
unesp.department | Análises Clínicas - FCF | pt |
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