A new complex of silver(I) with probenecid: Synthesis, characterization, and studies of antibacterial and extended spectrum β-lactamases (ESBL) inhibition activities

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dc.contributor.authorLustri, Wilton R.
dc.contributor.authorLazarini, Silmara C.
dc.contributor.authorSimei Aquaroni, Nayara Ap.
dc.contributor.authorResende, Flávia A.
dc.contributor.authorAleixo, Nadia A.
dc.contributor.authorPereira, Douglas H.
dc.contributor.authorLustri, Bruna Cardinali [UNESP]
dc.contributor.authorMoreira, Cristiano Gallina [UNESP]
dc.contributor.authorRibeiro, Camila M. [UNESP]
dc.contributor.authorPavan, Fernando R. [UNESP]
dc.contributor.authorNakahata, Douglas H.
dc.contributor.authorGonçalves, Adriano M. [UNESP]
dc.contributor.authorNascimento-Júnior, Nailton M. [UNESP]
dc.contributor.authorCorbi, Pedro P.
dc.contributor.institutionLaboratory of Bacterial Cellulose and Medicinal Chemistry (CBQUIM)
dc.contributor.institutionFederal University of Tocantins
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionDonostia International Physics Center
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:48:56Z
dc.date.available2023-07-29T13:48:56Z
dc.date.issued2023-06-01
dc.description.abstractThis article describes the in vitro antibacterial and β-lactamase inhibition of a novel silver(I) complex with the sulfonamide probenecid (Ag-PROB). The formula Ag2C26H36N2O8S2·2H2O for the Ag-PROB complex was proposed based on elemental analysis. High-resolution mass spectrometric studies revealed the existence of the complex in its dimeric form. Infrared, nuclear magnetic resonance spectroscopies and Density Functional Theory calculations indicated a bidentate coordination of probenecid to the silver ions by the oxygen atoms of the carboxylate. In vitro antibacterial activities of Ag-PROB showed significant growth inhibitory activity over Mycobacterium tuberculosis, S. aureus, and P. aeruginosa PA01biofilm-producers, B. cereus, and E. coli. The Ag-PROB complex was active over multi-drug resistant of uropathogenic E. coli extended spectrum β-lactamases (ESBL) producing (EC958 and BR43), enterohemorrhagic E. coli (O157:H7) and enteroaggregative E. coli (O104:H4). Ag-PROB was able to inhibit CTX-M-15 and TEM-1B ESBL classes, at concentrations below the minimum inhibitory concentration for Ag-PROB, in the presence of ampicillin (AMP) concentration in which EC958 and BR43 bacteria were resistant in the absence of Ag-PROB. These results indicate that, in addition to ESBL inhibition, there is a synergistic antibacterial effect between AMP and the Ag-PROB. Molecular docking results revealed potential key residues involved in interactions between Ag-PROB, CTX-M-15 and TEM[sbnd]1B, suggesting the molecular mechanism of the ESBL inhibition. The obtained results added to the absence of mutagenic activity and low cytotoxic activity over non-tumor cell of the Ag-PROB complex open a new perspective for future in vivo tests demonstrating its potential of use as an antibacterial agent.en
dc.description.affiliationDepartment of Biological and Health Sciences - University of Araraquara - UNIARA Laboratory of Bacterial Cellulose and Medicinal Chemistry (CBQUIM), São Paulo
dc.description.affiliationChemistry Collegiate Federal University of Tocantins, Campus Gurupi-Badejós, PO Box 66
dc.description.affiliationInstitute of Chemistry University of Campinas – UNICAMP, São Paulo
dc.description.affiliationDonostia International Physics Center, Paseo Manuel de Lardizabal 4
dc.description.affiliationSão Paulo State University – UNESP School of Pharmaceutical Sciences
dc.description.affiliationSão Paulo State University (Unesp) Institute of Chemistry Department of Biochemistry and Organic Chemistry Laboratory of Medicinal Chemistry Organic Synthesis and Molecular Modeling (LaQMedSOMM), São Paulo
dc.description.affiliationUnespSão Paulo State University – UNESP School of Pharmaceutical Sciences
dc.description.affiliationUnespSão Paulo State University (Unesp) Institute of Chemistry Department of Biochemistry and Organic Chemistry Laboratory of Medicinal Chemistry Organic Synthesis and Molecular Modeling (LaQMedSOMM), São Paulo
dc.description.sponsorshipASCRS Research Foundation
dc.description.sponsorshipIdASCRS Research Foundation: 2015/09833-0
dc.description.sponsorshipIdASCRS Research Foundation: 2015/20882-3
dc.description.sponsorshipIdASCRS Research Foundation: 2017/16278-9
dc.description.sponsorshipIdASCRS Research Foundation: 2017/19243-1
dc.description.sponsorshipIdASCRS Research Foundation: 2017/24914-2
dc.description.sponsorshipIdASCRS Research Foundation: 2018/00163-0
dc.description.sponsorshipIdASCRS Research Foundation: 2018/00187-7
dc.description.sponsorshipIdASCRS Research Foundation: 2018/02344-2
dc.description.sponsorshipIdASCRS Research Foundation: 2018/12062-4
dc.description.sponsorshipIdASCRS Research Foundation: 2018/12590-0
dc.description.sponsorshipIdASCRS Research Foundation: 2019/03049-7
dc.description.sponsorshipIdASCRS Research Foundation: 2019/26696-8
dc.description.sponsorshipIdASCRS Research Foundation: 2020/13497-4
dc.description.sponsorshipIdASCRS Research Foundation: 2021/07458-9
dc.description.sponsorshipIdASCRS Research Foundation: 2021/08717-8
dc.identifierhttp://dx.doi.org/10.1016/j.jinorgbio.2023.112201
dc.identifier.citationJournal of Inorganic Biochemistry, v. 243.
dc.identifier.doi10.1016/j.jinorgbio.2023.112201
dc.identifier.issn1873-3344
dc.identifier.issn0162-0134
dc.identifier.scopus2-s2.0-85151464550
dc.identifier.urihttp://hdl.handle.net/11449/248615
dc.language.isoeng
dc.relation.ispartofJournal of Inorganic Biochemistry
dc.sourceScopus
dc.subjectAntibacterial agent
dc.subjectMolecular docking
dc.subjectMulti-drug resistant
dc.subjectProbenecid
dc.subjectSilver complex
dc.subjectβ-lactamase inhibition
dc.titleA new complex of silver(I) with probenecid: Synthesis, characterization, and studies of antibacterial and extended spectrum β-lactamases (ESBL) inhibition activitiesen
dc.typeArtigo

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