Expression of heat shock protein in broiler embryo tissues after acute cold or heat stress

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dc.contributor.authorLeandro, N. S. M.
dc.contributor.authorGonzales, E.
dc.contributor.authorFerro, J. A.
dc.contributor.authorFerro, M. I. T.
dc.contributor.authorGivisiez, P. E. N.
dc.contributor.authorMacari, Marcos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:27:06Z
dc.date.available2014-05-20T15:27:06Z
dc.date.issued2004-02-01
dc.description.abstractThis study evaluated the expression of heat shock protein 70 kD (hsp70) in broiler chicken embryos subjected to cold (Experiment 1) or high incubation temperature (Experiment 11). In each experiment, fertile eggs were distributed in three incubators kept at 37.8degreesC. At day 13 (D13), D16, and D19 of incubation, the embryos were subjected to acute cold (32degreesC) or heat (40degreesC) for 4-6 hr. Immediately after cold or heat exposure, samples from the liver, heart, breast muscle, brain, and lungs of 40 embryos were taken per age and treatment (control or stressed embryos), A tissue pool from 10 embryos was used as 1 replication. The levels of hsp70 in each tissue sample was quantified by Western blot analysis. The data were analyzed in a 3 x 2 factorial arrangement of treatments with four replications. hsp70 was detected in all embryo tissues, and the brain contained 2- to 5-times more hsp70 protein compared to the other tissues in either cold or heat stressed embryos. hsp70 increases were observed in the heart and breast muscle of cold stressed embryos at D16 and D19, respectively. Heat stressed embryos showed an increase of hsp70 in the heart at D13 and D19, and in the lung at D19 of incubation. Younger embryos had higher hsp70 synthesis than older embryos, irrespective of the type of thermal stressor. The results indicate that the expression of hsp70 in broiler chicken embryos is affected by cold and heat distress, and is tissue- and age-dependent. (C) 2004 Wiley-Liss, Inc.en
dc.description.affiliationUNESP, Fac Vet & Zootecnia, BR-18618900 São Paulo, Brazil
dc.description.affiliationUNESP, Fac Ciências Agrarias & Vet, BR-18618900 São Paulo, Brazil
dc.description.affiliationUnespUNESP, Fac Vet & Zootecnia, BR-18618900 São Paulo, Brazil
dc.description.affiliationUnespUNESP, Fac Ciências Agrarias & Vet, BR-18618900 São Paulo, Brazil
dc.format.extent172-177
dc.identifierhttp://dx.doi.org/10.1002/mrd.10397
dc.identifier.citationMolecular Reproduction and Development. New York: Wiley-liss, v. 67, n. 2, p. 172-177, 2004.
dc.identifier.doi10.1002/mrd.10397
dc.identifier.issn1040-452X
dc.identifier.lattes0147241723612464
dc.identifier.lattes5713558572926669
dc.identifier.urihttp://hdl.handle.net/11449/37149
dc.identifier.wosWOS:000187889100006
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofMolecular Reproduction and Development
dc.relation.ispartofjcr3.113
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectHsp70pt
dc.subjectTemperaturapt
dc.subjectincubationpt
dc.subjectpoultrypt
dc.titleExpression of heat shock protein in broiler embryo tissues after acute cold or heat stressen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes0147241723612464
unesp.author.lattes5713558572926669
unesp.author.orcid0000-0002-6850-7145[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Morfologia e Fisiologia Animal - FCAV