Human dental pulp stem cell monolayer and spheroid therapy after spinal motor root avulsion in adult rats

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dc.contributor.authorPaes, Sabrina Moreira
dc.contributor.authorCastro, Mateus Vidigal de
dc.contributor.authorBarbosa, Rafael Maza
dc.contributor.authorPolitti Cartarozzi, Luciana
dc.contributor.authorCoser, Lilian de Oliveira
dc.contributor.authorKempe, Paula Regina Gelinski
dc.contributor.authorDecarli, Monize Caiado
dc.contributor.authorMoraes, Ângela Maria
dc.contributor.authorBarraviera, Benedito [UNESP]
dc.contributor.authorFerreira Júnior, Rui Seabra [UNESP]
dc.contributor.authorOliveira, Alexandre Leite Rodrigues de
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:36:11Z
dc.date.available2023-07-29T13:36:11Z
dc.date.issued2023-03-01
dc.description.abstractSpinal cord injuries result in severe neurological deficits and neuronal loss, with poor functional recovery. Mesenchymal stem cells have shown promising results; therefore the present objective of this work was to compare motor recovery after treatment with human dental pulp stem cells (hDPSC) cultivated in monolayer (2D) or as spheroids (3D), following avulsion and reimplantation of spinal motor roots in adult rats. Thus, 72 adult female Lewis rats were divided into 4 groups: avulsion (AV); avulsion followed by reimplantation (AR); avulsion associated with reimplant and 2D cell therapy (AR + 2D), and avulsion associated with reimplant and 3D cell therapy (AR + 3D). The application of the cells in 2D and 3D was performed by microsurgery, with subsequent functional assessment using a walking track test (Catwalk system), immunohistochemistry, neuronal survival, and qRT-PCR in 1-, 4-, and 12-weeks post-injury. The animals in the AR + 2D and AR + 3D groups showed the highest neuronal survival rates, and immunofluorescence revealed downregulation of GFAP, and Iba-1, with preservation of synaptophysin, indicating a reduction in glial reactivity, combined with the maintenance of pre-synaptic inputs. There was an increase in anti-inflammatory (IL-4, TGFβ) and a reduction of pro-inflammatory factors (IL-6, TNFα) in animals treated with reimplantation and hDPSC. As for the functional recovery, in all analyzed parameters, the AR + 2D group performed better and was superior to the avulsion alone. Overall, our results indicate that the 2D and 3D cell therapy approaches provide successful immunomodulation and motor recovery, consistent with advanced therapies after spinal cord injury.en
dc.description.affiliationLaboratory of Nerve Regeneration Institute of Biology (IB) State University of Campinas (UNICAMP), SP
dc.description.affiliationSchool of Chemical Engineering University of Campinas UNICAMP, Av. Albert Einstein, 500
dc.description.affiliationCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP), SP
dc.description.affiliationUnespCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP), SP
dc.description.sponsorshipUniversidade Estadual Paulista
dc.identifierhttp://dx.doi.org/10.1016/j.brainres.2022.148229
dc.identifier.citationBrain Research, v. 1802.
dc.identifier.doi10.1016/j.brainres.2022.148229
dc.identifier.issn1872-6240
dc.identifier.issn0006-8993
dc.identifier.scopus2-s2.0-85145996529
dc.identifier.urihttp://hdl.handle.net/11449/248162
dc.language.isoeng
dc.relation.ispartofBrain Research
dc.sourceScopus
dc.subjectCell therapy
dc.subjectDental pulp
dc.subjectSpheroid
dc.subjectSpinal cord injury
dc.titleHuman dental pulp stem cell monolayer and spheroid therapy after spinal motor root avulsion in adult ratsen
dc.typeArtigo

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Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos