Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests

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dc.contributor.authorPrudencio, Carlos Roberto
dc.contributor.authorGomes da Costa, Vivaldo [UNESP]
dc.contributor.authorRocha, Leticia Barboza
dc.contributor.authorda Costa, Hernan Hermes Monteiro
dc.contributor.authorOrts, Diego José Belato
dc.contributor.authorda Silva Santos, Felipe Rocha
dc.contributor.authorRahal, Paula [UNESP]
dc.contributor.authorLino, Nikolas Alexander Borsato [UNESP]
dc.contributor.authorda Conceição, Pâmela Jóyce Previdelli [UNESP]
dc.contributor.authorBittar, Cintia [UNESP]
dc.contributor.authorMachado, Rafael Rahal Guaragna
dc.contributor.authorDurigon, Edison Luiz
dc.contributor.authorAraujo, João Pessoa [UNESP]
dc.contributor.authorPolatto, Juliana Moutinho
dc.contributor.authorda Silva, Miriam Aparecida
dc.contributor.authorde Oliveira, Joyce Araújo
dc.contributor.authorMitsunari, Thais
dc.contributor.authorPereira, Lennon Ramos
dc.contributor.authorAndreata-Santos, Robert
dc.contributor.authorde Souza Ferreira, Luís Carlos
dc.contributor.authorLuz, Daniela
dc.contributor.authorPiazza, Roxane Maria Fontes
dc.contributor.institutionInstituto Adolfo Lutz
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionInstituto Butantan
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2023-07-29T16:10:56Z
dc.date.available2023-07-29T16:10:56Z
dc.date.issued2023-03-01
dc.description.abstractZika virus (ZIKV), a mosquito-borne pathogen, is an emerging arbovirus associated with sporadic symptomatic cases of great medical concern, particularly among pregnant women and newborns affected with neurological disorders. Serological diagnosis of ZIKV infection is still an unmet challenge due to the co-circulation of the dengue virus, which shares extensive sequence conservation of structural proteins leading to the generation of cross-reactive antibodies. In this study, we aimed to obtain tools for the development of improved serological tests for the detection of ZIKV infection. Polyclonal sera (pAb) and a monoclonal antibody (mAb 2F2) against a recombinant form of the ZIKV nonstructural protein 1 (NS1) allowed the identification of linear peptide epitopes of the NS1 protein. Based on these findings, six chemically synthesized peptides were tested both in dot blot and ELISA assays using convalescent sera collected from ZIKV-infected patients. Two of these peptides specifically detected the presence of ZIKV antibodies and proved to be candidates for the detection of ZIKV-infected subjects. The availability of these tools opens perspectives for the development of NS1-based serological tests with enhanced sensitivity regarding other flaviviruses.en
dc.description.affiliationLaboratório de Imunobiotecnologia Centro de Imunologia Instituto Adolfo Lutz, Av. Dr. Arnaldo, 351, SP
dc.description.affiliationInstituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Cristóvão Colombo, 2265, SP
dc.description.affiliationLaboratório de Bacteriologia Instituto Butantan, Av. Vital Brazil, 1500, SP
dc.description.affiliationDepartamento de Microbiologia Instituto de Ciências Biomédicas Universidade de São Paulo, SP
dc.description.affiliationInstituto de Biotecnologia Universidade Estadual Paulista Júlio de Mesquita Filho, SP
dc.description.affiliationPlataforma Científica Pasteur USP Universidade de São Paulo, SP
dc.description.affiliationUnespInstituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Cristóvão Colombo, 2265, SP
dc.description.affiliationUnespInstituto de Biotecnologia Universidade Estadual Paulista Júlio de Mesquita Filho, SP
dc.description.sponsorshipFundação Butantan
dc.description.sponsorshipIdFundação Butantan: 2015/001
dc.identifierhttp://dx.doi.org/10.3390/v15030654
dc.identifier.citationViruses, v. 15, n. 3, 2023.
dc.identifier.doi10.3390/v15030654
dc.identifier.issn1999-4915
dc.identifier.scopus2-s2.0-85152253473
dc.identifier.urihttp://hdl.handle.net/11449/249851
dc.language.isoeng
dc.relation.ispartofViruses
dc.sourceScopus
dc.subjectdiagnosis
dc.subjectepitopes
dc.subjectimmunoassays
dc.subjectmonoclonal antibody
dc.subjectNS1 protein
dc.subjectpeptides
dc.subjectZika virus
dc.titleIdentification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Testsen
dc.typeArtigo
unesp.author.orcid0000-0001-7701-0707[1]
unesp.author.orcid0000-0002-9359-0198[2]
unesp.author.orcid0000-0001-8972-749X[4]
unesp.author.orcid0000-0003-4209-9005[5]
unesp.author.orcid0000-0002-8392-1467[6]
unesp.author.orcid0000-0002-8146-723X[8]
unesp.author.orcid0000-0002-2048-4589[10]
unesp.author.orcid0000-0001-6974-5092[11]
unesp.author.orcid0000-0003-4898-6553[12]
unesp.author.orcid0000-0002-9153-1485[13]
unesp.author.orcid0000-0002-6143-0704[16]
unesp.author.orcid0000-0002-8070-7772[18]
unesp.author.orcid0000-0002-4506-3756[19]
unesp.author.orcid0000-0002-4883-1693[20]
unesp.author.orcid0000-0001-9622-0320[21]
unesp.author.orcid0000-0003-0068-8506[22]

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