Creatine supplementation and oxidative stress in rat liver

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dc.contributor.authorAraujo, Michel B. [UNESP]
dc.contributor.authorMoura, Leandro P. [UNESP]
dc.contributor.authorVieira Junior, Roberto C.
dc.contributor.authorJunior, Marcelo C. [UNESP]
dc.contributor.authorDalia, Rodrigo A. [UNESP]
dc.contributor.authorSponton, Amanda C. [UNESP]
dc.contributor.authorRibeiro, Carla [UNESP]
dc.contributor.authorMello, Maria Alice R. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Fed Mato Grosso
dc.date.accessioned2014-12-03T13:11:04Z
dc.date.available2014-12-03T13:11:04Z
dc.date.issued2013-12-10
dc.description.abstractBackground: The objective of this study was to determine the effects of creatine supplementation on liver biomarkers of oxidative stress in exercise-trained rats.Methods: Forty 90-day-old adult male Wistar rats were assigned to four groups for the eight-week experiment. Control group (C) rats received a balanced control diet; creatine control group (CCr) rats received a balanced diet supplemented with 2% creatine; trained group (T) rats received a balanced diet and intense exercise training equivalent to the maximal lactate steady state phase; and supplemented-trained (TCr) rats were given a balanced diet supplemented with 2% creatine and subjected to intense exercise training equivalent to the maximal lactate steady state phase. At the end of the experimental period, concentrations of creatine, hydrogen peroxide (H2O2) and thiobarbituric acid reactive substances (TBARS) were measured as well as the enzyme activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-GPx) and catalase (CAT). Liver tissue levels of reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio were also determined.Results: Hepatic creatine levels were highest in the CCr and TCr groups with increased concentration of H2O2 observed in the T and TCr animal groups. SOD activity was decreased in the TCr group. GSH-GPx activity was increased in the T and TCr groups while CAT was elevated in the CCr and TCr groups. GSH, GGS and the GSH/GSSG ratio did not differ between all animal subsets.Conclusions: Our results demonstrate that creatine supplementation acts in an additive manner to physical training to raise antioxidant enzymes in rat liver. However, because markers of liver oxidative stress were unchanged, this finding may also indicate that training-induced oxidative stress cannot be ameliorated by creatine supplementation.en
dc.description.affiliationUniv Estadual Paulista, Dept Phys Educ, Lab Nutr Metab & Exercise, Rio Claro, SP, Brazil
dc.description.affiliationUniv Fed Mato Grosso, UFMT, Fac Nutr, Postgrad Program Graduat Biosci, Culaba, Mato Grosso, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Phys Educ, Lab Nutr Metab & Exercise, Rio Claro, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 09/52063-0
dc.format.extent8
dc.identifierhttp://dx.doi.org/10.1186/1550-2783-10-54
dc.identifier.citationJournal Of The International Society Of Sports Nutrition. London: Biomed Central Ltd, v. 10, 8 p., 2013.
dc.identifier.doi10.1186/1550-2783-10-54
dc.identifier.fileWOS000328961100001.pdf
dc.identifier.issn1550-2783
dc.identifier.urihttp://hdl.handle.net/11449/112800
dc.identifier.wosWOS:000328961100001
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofJournal of the International Society of Sports Nutrition
dc.relation.ispartofjcr3.135
dc.relation.ispartofsjr0,775
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectCreatine supplementationen
dc.subjectOxidative stressen
dc.subjectEnzymesen
dc.subjectTreadmill exerciseen
dc.titleCreatine supplementation and oxidative stress in rat liveren
dc.typeArtigo
dcterms.rightsHolderBiomed Central Ltd
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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