An immunocytochemical and in situ hybridization analysis of annexin 1 expression in rat mast cells: Modulation by inflammation and dexamethasone

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dc.contributor.authorOliani, Sonia M. [UNESP]
dc.contributor.authorChristian, Helen C.
dc.contributor.authorManston, John
dc.contributor.authorFlower, Roderick J.
dc.contributor.authorPerretti, Mauro
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionSt. Bartholomew's and the Royal London School of Medicine and Dentistry
dc.contributor.institutionUniversity of Oxford
dc.contributor.institutionQueen Mary and Westfield College
dc.date.accessioned2022-04-28T19:54:57Z
dc.date.available2022-04-28T19:54:57Z
dc.date.issued2000-01-01
dc.description.abstractThe presence and localization of the anti-inflammatory protein annexin 1 (also known as lipocortin 1) in perivenular rat mast cells was investigated here. Using the rat mesenteric microvascular bed and a combination of morphologic techniques ranging from immunofluorescence to electron microscopy analyses, we detected the presence of annexin 1 in discrete intracellular sites, both in the nucleus and in the cytoplasm. In resting mast cells, most of the protein pool (approximately 80% of the cytosolic portion) was localized to cytoplasmic granules. In agreement with other cell types, treatment of rats with dexamethasone (0.2 mg/kg, ip) increased annexin 1 expression in mast cells, inducing a remarkable appearance of clusters of protein immunoreactivity. This effect was most likely the result of de novo protein synthesis as determined by an increase in mRNA seen by in situ hybridization. Triggering an ongoing experimental inflammatory response (0.3 mg of carrageenin, ip) increased annexin 1 mRNA and protein levels. In conclusion, we report for the first time the localization of annexin 1 in connective tissue mast cells, and its susceptibility not only to glucocorticoid hormone treatment, but also to an experimental acute inflammatory response.en
dc.description.affiliationDepartment of Biology IBILCE UNESP, São José do Rio Preto
dc.description.affiliationDepartment of Biochemical Pharmacology William Harvey Research Institute St. Bartholomew's and the Royal London School of Medicine and Dentistry, London
dc.description.affiliationDepartment of Human Anatomy and Genetics University of Oxford, Oxford
dc.description.affiliationDepartment of Neuroscience Queen Mary and Westfield College, London
dc.description.affiliationUnespDepartment of Biology IBILCE UNESP, São José do Rio Preto
dc.format.extent1429-1438
dc.identifierhttp://dx.doi.org/10.1038/labinvest.3780150
dc.identifier.citationLaboratory Investigation, v. 80, n. 9, p. 1429-1438, 2000.
dc.identifier.doi10.1038/labinvest.3780150
dc.identifier.issn0023-6837
dc.identifier.scopus2-s2.0-0033833210
dc.identifier.urihttp://hdl.handle.net/11449/224155
dc.language.isoeng
dc.relation.ispartofLaboratory Investigation
dc.sourceScopus
dc.titleAn immunocytochemical and in situ hybridization analysis of annexin 1 expression in rat mast cells: Modulation by inflammation and dexamethasoneen
dc.typeArtigo

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