Atividade anti Staphylococcus aureus de extratos de própolis (EP) de Apis mellifera preparados com diferentes concentrações de etanol

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Fernandas, A. [UNESP]
Balestrin, E. C D [UNESP]
Cunha, M. L R S [UNESP]

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Propolis has been used in folk medicine and possesses a broad spectrum of biological activities, specially antibacterial activity. Studies have demonstrated that the composition of propolis extract may have influence in such activity. The goal of this study was to investigate the antibacterial activity of eleven propolis extracts (PE) against sixty one Staphylococcus aureus strains, isolated from newborn clinical specimens. The PE from Apis mellifera were prepared by using pure water and mixtures of water with ethanol at different concentrations (from 0 to 100%), 25g of propolis in 100 mL of solvent, and three days of maceration followed by filtration. Determination of Minimal Inhibitory Concentration (MIC) by agar dilution method was performed and serial concentrations from each PE were achieved (%v/v) in plates containing Mueller Hinton agar. It was possible to verify that the anti S. aureus activity was directly proportional to ethanol concentration and no significant differences were observed among PE with ethanol concentration from 70 to 100%. The MIC 90% values ranged from 0.4 to 0.6% (v/v) and the 70% ethanolic extract were the most efficient to inhibit bacterial growth (MIC 90%=0.42%, v/v). In conclusion, our results suggest that the EP composition and, consequently, the concentration of ethanol used as solvent may influence the antibacterial activity of propolis from A. mellifera.



Apis mellifera, Aqueous Extract, Ethanolic Extract, Minimal Inhibitory Concentration (MIC), Propolis, Staphylococcus aureus, agar, alcohol, bee propolis extract, Mueller Hinton agar, propolis, unclassified drug, water, agar dilution method, animal product, antibacterial activity, antistaphylococcal activity, bacterial growth, chemical composition, filtration, honeybee, minimum inhibitory concentration, newborn infection, nonhuman, technique, traditional medicine

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Revista de Ciencias Farmaceuticas, v. 24, n. 2, p. 147-152, 2004.