The incidence of Shiga toxin-producing Escherichia coli in cattle with mastitis in Brazil

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dc.contributor.authorLira, W. M.
dc.contributor.authorMacedo, C.
dc.contributor.authorMarin, J. M.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T15:20:03Z
dc.date.available2014-05-20T15:20:03Z
dc.date.issued2004-01-01
dc.description.abstractAims: To determine the prevalence and molecular characteristics of Shiga toxin-producing Escherichia coli (STEC) isolates from bovine mastitic milk in Brazil.Methods and Results: A total of 2144 milk samples from dairy cattle showing mastitis were screened for the presence of E. coli. A total of 182 E. coli isolates were selected and examined. All were subjected to dot blot analysis using the CVD419 probe for the detection of the enterohaemolysin (hly) gene, and to a multiplex PCR for the detection of stx1, stx2 and eaeA genes. STEC were isolated from 22 (12.08%) milk samples. All the STEC isolates were tested for sensibility to 10 antimicrobials; the resistances most commonly observed were to cephalothin (86.3%), tetracycline (63.6%) and doxycycline (63.6%).Conclusion: STEC isolates were found in bovine mastitic milk in Brazil.Significance and Impact of the Study: STEC isolates from mastitic milk were potentially pathogenic for human in that they belonged to serogroups associated with diarrhoea and haemolytic-uraemic syndrome, some of them were stx2, eaeA and hly positive.en
dc.description.affiliationUniv Estadual Paulista, Fac Ciências Agr & Vet Jaboticabal, Dept Microbiol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Dept Genet, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Morfol Estomatol & Fisiol FORP, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências Agr & Vet Jaboticabal, Dept Microbiol, São Paulo, Brazil
dc.format.extent861-866
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2672.2004.02384.x
dc.identifier.citationJournal of Applied Microbiology. Oxford: Blackwell Publishing Ltd, v. 97, n. 4, p. 861-866, 2004.
dc.identifier.doi10.1111/j.1365-2672.2004.02384.x
dc.identifier.fileWOS000223817600022.pdf
dc.identifier.issn1364-5072
dc.identifier.urihttp://hdl.handle.net/11449/31411
dc.identifier.wosWOS:000223817600022
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofJournal of Applied Microbiology
dc.relation.ispartofjcr2.160
dc.relation.ispartofsjr0,795
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectdairy cattlept
dc.subjectEscherichia colipt
dc.subjectmastitispt
dc.subjectShiga toxin-producing E. colipt
dc.titleThe incidence of Shiga toxin-producing Escherichia coli in cattle with mastitis in Brazilen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderBlackwell Publishing Ltd

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