Detection of intestinal parasites in human faecal samples using dissolved air flotation

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dc.contributor.authorSoares, Felipe Augusto
dc.contributor.authordos Santos, Bianca Martins
dc.contributor.authorRosa, Stefany Laryssa
dc.contributor.authorLoiola, Saulo Hudson Nery
dc.contributor.authorStéfano, Vitória Castilho
dc.contributor.authorProença, Letícia Rodrigues
dc.contributor.authorSuzuki, Celso Tetsuo Nagase
dc.contributor.authorSabadini, Edvaldo
dc.contributor.authorBresciani, Katia Denise Saraiva [UNESP]
dc.contributor.authorFalcão, Alexandre Xavier
dc.contributor.authorGomes, Jancarlo Ferreira
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionImmunoCamp Science and Technology
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:59:40Z
dc.date.available2023-07-29T13:59:40Z
dc.date.issued2022-12-01
dc.description.abstractObjective: Ova and parasite (O&P) examination is recommended for the laboratory diagnosis of agents causing parasitic infections; however, this exam requires scientific and technological improvements to enhance its diagnostic validity. Dissolved air flotation (DAF) is an efficient technical principle separating suspended solids in a liquid medium. We aimed to develop and validate a new procedure for intestinal parasite detection with DAF. Methods: In this study, we collected samples from 500 volunteers, screened them by direct examination, and transferred the material to tubes using the Three Faecal Test (TF-Test) for triplicate DAF tests. We evaluated physical–chemical parameters and DAF prototype components through quantifying parasites recovered from floated and non-floated regions of the flotation column. The DAF operation protocol was validated with the gold standard results. Results: The 10% saturated volume proportion and cationic surfactant showed regularity and high parasite recovery (80%). Modifications of the needle device did not influence parasite recovery (p > 0.05). Sensitivity, specificity, accuracy and kappa agreement obtained with the DAF protocol were 91%, 100%, 93% and substantial (k = 0.64), respectively. Conclusion: The DAF principle could be used to process faecal samples in routine laboratory exams, enabling intestinal parasite detection.en
dc.description.affiliationSchool of Medical Sciences University of Campinas, São Paulo
dc.description.affiliationInstitute of Computing (IC) Laboratory of Image Data Science (LIDS) University of Campinas, São Paulo
dc.description.affiliationImmunoCamp Science and Technology, São Paulo
dc.description.affiliationInstitute of Chemistry Department of Physics-Chemistry University of Campinas, São Paulo
dc.description.affiliationSchool of Veterinary Medicine São Paulo State University, São Paulo
dc.description.affiliationUnespSchool of Veterinary Medicine São Paulo State University, São Paulo
dc.format.extent1044-1052
dc.identifierhttp://dx.doi.org/10.1111/tmi.13827
dc.identifier.citationTropical Medicine and International Health, v. 27, n. 12, p. 1044-1052, 2022.
dc.identifier.doi10.1111/tmi.13827
dc.identifier.issn1365-3156
dc.identifier.issn1360-2276
dc.identifier.scopus2-s2.0-85141379174
dc.identifier.urihttp://hdl.handle.net/11449/248999
dc.language.isoeng
dc.relation.ispartofTropical Medicine and International Health
dc.sourceScopus
dc.subjectdiagnostic techniques and procedures
dc.subjectflotation
dc.subjectparasitic diseases
dc.subjectparasitology
dc.titleDetection of intestinal parasites in human faecal samples using dissolved air flotationen
dc.typeArtigo
unesp.author.orcid0000-0001-6262-5194[1]

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Artigos - Físico Química - IQ