The intricate role of mast cell proteases and the annexin A1-FPR1 system in abdominal wall endometriosis

dc.contributor.authorPaula Júnior, Rubens [UNESP]
dc.contributor.authorOliani, Antonio Hélio
dc.contributor.authorVaz-Oliani, Denise Cristina Mos
dc.contributor.authorD'Ávila, Solange Corrêa Garcia Pires
dc.contributor.authorOliani, Sonia Maria [UNESP]
dc.contributor.authorGil, Cristiane Damas
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFaculdade de Medicina de São José do Rio Preto (FAMERP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-04-27T11:56:05Z
dc.date.available2015-04-27T11:56:05Z
dc.date.issued2014
dc.description.abstractEndometriosis is a continuous and progressive disease with a poorly understood aetiology, pathophysiology and natural history. This study evaluated the histological differences between eutopic and ectopic endometria (abdominal wall endometriosis) and the expression of mast cell proteases (tryptase and chymase), annexin A1 (ANXA1) and formyl peptide receptor 1 (FPR1). Ectopic endometrium from 18 women with abdominal wall endometriosis and eutopic endometrium from 10 women without endometriosis were obtained. The endometrial samples were analysed by histopathology, immunohistochemistry and ultrastructural immunogold labeling to determine mast cell heterogeneity (tryptase and chymase positive cells) and the expression levels of ANXA1 and FPR1. Histopathological analysis of the endometriotic lesions showed a glandular pattern of mixed differentiation and an undifferentiated morphology with a significant influx of inflammatory cells and a change in mast cell heterogeneity, as evidenced by a significant increase in the number of chymase-positive cells and endogenous chymase expression. The undifferentiated glandular pattern of endometriotic lesions was positively associated with a marked increase and co-localization of ANXA1 and FPR1 in the epithelial cells. In conclusion, the co-upregulated expression of mast cell chymase and ANXA1–FPR1 system in ectopic endometrium suggests their involvement in the development of endometriotic lesions.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Biologia, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, Sao Jose do Rio Preto, Rua Cristovão Colombo, 2265 (Laboratório de Morfologia), Jardim Nazareth, CEP 15054-000, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Biologia, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto
dc.format.extent34-43
dc.identifierhttp://link.springer.com/article/10.1007%2Fs10735-014-9595-y
dc.identifier.citationJournal of Molecular Histology, v. 46, n. 1, p. 34-43, 2014.
dc.identifier.issn1567-2387
dc.identifier.lattes5102737730539655
dc.identifier.urihttp://hdl.handle.net/11449/122839
dc.language.isoeng
dc.relation.ispartofJournal of Molecular Histology
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectChymaseen
dc.subjectEndometriomaen
dc.subjectImmunohistochemistryen
dc.subjectInflammationen
dc.subjectLipocortin-1en
dc.subjectTransmission electron microscopyen
dc.titleThe intricate role of mast cell proteases and the annexin A1-FPR1 system in abdominal wall endometriosisen
dc.typeArtigo
unesp.author.lattes5102737730539655
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentBiologiapt

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