The kinetics of donor cell mtDNA in embryonic and somatic donor cell-derived bovine embryos

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dc.contributor.authorFerreira, Christina Ramires [UNESP]
dc.contributor.authorMeirelles, Flavio Vieira
dc.contributor.authorYamazaki, Walt [UNESP]
dc.contributor.authorChiaratti, Marcos Roberto
dc.contributor.authorMeo, Simone Cristina
dc.contributor.authorPerecin, Felipe [UNESP]
dc.contributor.authorSmith, Lawrence Charles
dc.contributor.authorGarcia, Joaquim Mansano [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInst Zootecnia
dc.contributor.institutionUniv Montreal
dc.date.accessioned2014-05-20T13:15:12Z
dc.date.available2014-05-20T13:15:12Z
dc.date.issued2007-12-01
dc.description.abstractThe mechanisms controlling the outcome of donor cell-derived mitochondrial DNA (mtDNA) in cloned animals remain largely unknown. This research was designed to investigate the kinetics of somatic and embryonic mtDNA in reconstructed bovine embryos during preimplantation development, as well as in cloned animals. The experiment involved two different procedures of embryo reconstruction and their evaluation at five distinct phases of embryo development to measure the proportion of donor cell mtDNA (Bos indicus), as well as the segregation of this mtDNA during cleavage. The ratio of donor cell (B. indicus) to host oocyte (B. taurus) mtDNA (heteroplasmy) from blastomere- (NT-B) and fibroblast- (NT-F) reconstructed embryos was estimated using an allele-specific PCR with fluorochrome-stained specific primers in each sampled blastomere, in whole blastocysts, and in the tissues of a fibroblast-derived newborn clone. NT-B zygotes and blastocysts show similar levels of heteroplasmy (11.0% and 14.0%, respectively), despite a significant decrease at the 9-16 cell stage (5.8%; p < 0.05). Heteroplasmy levels in NT-F reconstructed zygotes, however, increased from an initial low level (4.7%), to 12.9% (p < 0.05) at the 9-16 cell stage. The NT-F blastocysts contained low levels of heteroplasmy (2.2%) and no somatic-derived mtDNA was detected in the gametes or the tissues of the newborn calf cloned. These results suggest that, in contrast to the mtDNA of blastomeres, that of somatic cells either undergoes replication or escapes degradation during cleavage, although it is degraded later after the blastocyst stage or lost during somatic development, as revealed by the lack of donor cell mtDNA at birth.en
dc.description.affiliationFCAV UNESP, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, SP, Brazil
dc.description.affiliationFZEA USP, Dept Ciências Basicas, Pirassununga, SP, Brazil
dc.description.affiliationInst Zootecnia, Nova Odessa, SP, Brazil
dc.description.affiliationUniv Montreal, CRRA, St Hyacinthe, PQ, Canada
dc.description.affiliationUnespFCAV UNESP, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, SP, Brazil
dc.format.extent618-629
dc.identifierhttp://dx.doi.org/10.1089/clo.2006.0082
dc.identifier.citationCloning and Stem Cells. New Rochelle: Mary Ann Liebert Inc., v. 9, n. 4, p. 618-629, 2007.
dc.identifier.doi10.1089/clo.2006.0082
dc.identifier.fileWOS000252027800016.pdf
dc.identifier.issn1536-2302
dc.identifier.lattes2251116139872527
dc.identifier.urihttp://hdl.handle.net/11449/2405
dc.identifier.wosWOS:000252027800016
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc.
dc.relation.ispartofCloning and Stem Cells
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleThe kinetics of donor cell mtDNA in embryonic and somatic donor cell-derived bovine embryosen
dc.typeArtigo
dcterms.licensehttp://www.liebertpub.com/nv/resources-tools/self-archiving-policy/51/
dcterms.rightsHolderMary Ann Liebert Inc.
unesp.author.lattes2251116139872527
unesp.author.orcid0000-0001-8805-9469[4]
unesp.author.orcid0000-0003-0372-4920[2]
unesp.author.orcid0000-0003-0046-0050[5]
unesp.author.orcid0000-0003-3988-6454[1]
unesp.author.orcid0000-0003-2009-5863[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentMedicina Veterinária Preventiva e Reprodução Animal - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias