Nanocarrier-Mediated Delivery of miRNA, RNAi, and CRISPR-Cas for Plant Protection: Current Trends and Future Directions

dc.contributor.authorMujtaba, Muhammad
dc.contributor.authorWang, Depeng
dc.contributor.authorCarvalho, Lucas Bragança [UNESP]
dc.contributor.authorOliveira, Jhones Luiz [UNESP]
dc.contributor.authorEspirito Santo Pereira, Anderson Do [UNESP]
dc.contributor.authorSharif, Rahat
dc.contributor.authorJogaiah, Sudisha
dc.contributor.authorPaidi, Murali Krishna
dc.contributor.authorWang, Lichen
dc.contributor.authorAli, Qasid
dc.contributor.authorFraceto, Leonardo Fernandes [UNESP]
dc.contributor.institutionAalto University
dc.contributor.institutionAnkara University
dc.contributor.institutionLinyi University
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionYangzhou University
dc.contributor.institutionKarnatak University
dc.contributor.institutionCSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI)
dc.contributor.institutionAkdeniz University
dc.description.abstractCurrent trends in plant genetic transformation technologies, i.e., designing and applying molecules like miRNA, RNAi, and CRISPR-Cas, largely enable researchers to target specific sites in the plant genome to avert the growing biotic and abiotic threats to plants. However, the delivery of these molecules through conventional techniques brings an array of drawbacks such as low efficiency due to the cell wall barrier, tissue damage that leads to browning or necrosis, degradation of these biomolecules by physiological conditions (high temperature, harsh pH, and light), and plant-specific protocols. The advancements in nanotechnology offer an excellent alternative for the safe and highly efficient delivery of biomolecules such as miRNA, CRISPR-Cas, and RNAi without damaging the plant tissues. Nanoparticle (polymeric, metallic, magnetic, silica, carbon, etc.)-based delivery of biomolecules can be efficiently utilized especially for plant protection applications. Herein, we present a comprehensive overview of current trends (with a focus on the previous five years) in nanoparticle-based delivery of miRNA, RNAi, CRISPR-Cas and simillar biomolecules for plant protection applications. In addition, a future perspective focuses on the research gaps and unexplored potentials of nanoparticles for the delivery of biomolecules.en
dc.description.affiliationDepartment of Bioproducts and Biosystems School of Chemical Engineering Aalto University
dc.description.affiliationInstitute of Biotechnology Ankara University
dc.description.affiliationCollege of Life Science Linyi University, Shandong
dc.description.affiliationDepartment of Environmental Engineering Institute of Science and Technology of Sorocaba São Paulo State University (UNESP)
dc.description.affiliationSchool of Horticulture and Plant Protection Yangzhou University
dc.description.affiliationLaboratory of Plant Healthcare and Diagnostics P. G. Department of Biotechnology and Microbiology Karnatak University, Karnataka
dc.description.affiliationAcSIR CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Gijubhai Badheka Marg, Gujarat
dc.description.affiliationDepartment of Horticulture Faculty of Agriculture Akdeniz University
dc.description.affiliationUnespDepartment of Environmental Engineering Institute of Science and Technology of Sorocaba São Paulo State University (UNESP)
dc.identifier.citationACS Agricultural Science and Technology.
dc.relation.ispartofACS Agricultural Science and Technology
dc.subjectcontrolled delivery
dc.subjectDNA origami
dc.subjectplant protection
dc.titleNanocarrier-Mediated Delivery of miRNA, RNAi, and CRISPR-Cas for Plant Protection: Current Trends and Future Directionsen
dc.typeResenha 0000-0001-8392-9226[1][5]