Circulating leukocyte heat shock protein 70 (HSP70) and oxidative stress markers in rats after a bout of exhaustive exercise

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dc.contributor.authorAntunes-Neto, J. M. F.
dc.contributor.authorToyama, M. H.
dc.contributor.authorCarneiro, E. M.
dc.contributor.authorBoschero, A. C.
dc.contributor.authorPereira-Da-Silva, L.
dc.contributor.authorMacedo, D. V.
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionLab Estudos Multidisciplinares Estresse
dc.date.accessioned2014-05-20T13:12:16Z
dc.date.available2014-05-20T13:12:16Z
dc.date.issued2006-06-01
dc.description.abstractA novel method to measure oxidative stress resulting from exhaustive exercise in rats is presented. In this new procedure we evaluated the erythrocyte antioxidant enzymes, catalase ( CAT) and glutathione reductase (GR), the plasma oxidative attack markers, reactive carbonyl derivatives (RCD) and thiobarbituric reactive substances (TBARS). Muscular tissue damage was evaluated by monitoring plasma creatine kinase (CK) and plasma taurine ( Tau) concentrations. Also, we monitored total sulphydryl groups (TSG) and uric acid (UA), and the level of the 70 kDa heat shock protein (HSP70) in leukocytes as a marker of oxidative stress. In the study we found a correspondence between erythrocyte CAT and GR activities and leukocyte HSP70 levels, principally 3 h after the acute exercise, and this suggested an integrated mechanism of antioxidant defense. The increase in levels of plasma Tau was coincident with the increasing plasma levels of CK and TBARS, principally after two hours of exercise. Thus tissue damage occurred before the expression of any anti-oxidant system markers and the monitoring of Tau, CK or TBARS may be important for the estimation of oxidative stress during exhaustive exercise. Furthermore, the integrated analyses could be of value in a clinical setting to quantify the extent of oxidative stress risk and reduce the need to perform muscle biopsies as a tool of clinical evaluation.en
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Dept Bioquim, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Dept Fisiol & Biofis, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUNESP, Unidade Sao Vicente, Sao Vicente, Brazil
dc.description.affiliationLab Estudos Multidisciplinares Estresse, São Paulo, Brazil
dc.description.affiliationUnespUNESP, Unidade Sao Vicente, Sao Vicente, Brazil
dc.format.extent107-115
dc.identifierhttp://dx.doi.org/10.1080/10253890600772211
dc.identifier.citationStress-the International Journal on the Biology of Stress. Abingdon: Taylor & Francis Ltd, v. 9, n. 2, p. 107-115, 2006.
dc.identifier.doi10.1080/10253890600772211
dc.identifier.issn1025-3890
dc.identifier.lattes8573195327542061
dc.identifier.urihttp://hdl.handle.net/11449/257
dc.identifier.wosWOS:000241211600005
dc.language.isoeng
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofStress-the International Journal on the Biology of Stress
dc.relation.ispartofjcr3.047
dc.relation.ispartofsjr1,260
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectexercisept
dc.subjectfree amino acidspt
dc.subjectheat shock proteinpt
dc.subjectoxidative stresspt
dc.titleCirculating leukocyte heat shock protein 70 (HSP70) and oxidative stress markers in rats after a bout of exhaustive exerciseen
dc.typeArtigo
dcterms.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dcterms.rightsHolderTaylor & Francis Ltd
unesp.author.lattes8573195327542061
unesp.author.orcid0000-0003-3829-8570[4]
unesp.author.orcid0000-0001-8812-8954[6]
unesp.author.orcid0000-0001-6836-3084[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, São Vicentept

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Artigos - Ciências Biológicas - São Vicente