Identification of Rv3852 as a nucleoid-associated protein in Mycobacterium tuberculosis

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dc.contributor.authorWerlang, Isabel C. R.
dc.contributor.authorSchneider, Cristopher Z.
dc.contributor.authorMendonca, Jordana D.
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.authorBasso, Luiz A.
dc.contributor.authorSantos, Diogenes S.
dc.contributor.institutionPontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
dc.contributor.institutionUniversidade Federal do Rio Grande do Sul (UFRGS)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:55:11Z
dc.date.available2014-05-20T13:55:11Z
dc.date.issued2009-08-01
dc.description.abstractTuberculosis remains the major cause of mortality due to a bacterial pathogen, Mycobacterium tuberculosis. The molecular mechanisms of infection and persistence have not been completely elucidated for this pathogen. Studies involving nucleoid-associated proteins (NAPs), which have been related to the control and influence of virulence genes in pathogenic bacteria, can help unveil the virulence process of M. tuberculosis. Here, we describe the initial characterization of an ORF for an M. tuberculosis putative NAP. The Rv3852 gene was cloned and expressed, and its product purified to homogeneity. A qualitative protein-DNA binding assay was carried out by gel-retardation and the protein affinity for specific DNA sequences was assessed quantitatively by surface plasmon resonance (SPR). A stoichiometry of 10 molecules of monomeric protein per molecule of DNA was determined. The monophasic apparent dissociation rate constant values increased to a saturable level as a function of protein concentration, yielding two limiting values for the molecular recognition of proU2 DNA. A protein-DNA binding mechanism is proposed. In addition, functional complementation studies with an Escherichia coli has mutant reinforce the likelihood that the Rv3852 protein represents a novel NAP in M. tuberculosis.en
dc.description.affiliationPontificia Univ Catolica Rio Grande do Sul, Inst Nacl Ciência & Tecnol Tuberculose, Ctr Pesquisas Biol Mol & Func, BR-90619900 Porto Alegre, RS, Brazil
dc.description.affiliationUniversidade Federal do Rio Grande do Sul (UFRGS), Programa Posgrad Biol Celular & Mol, BR-91501970 Porto Alegre, RS, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Ctr Estudos Insetos Sociais, Lab Biol Estrutural & Zooquim,Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Ctr Estudos Insetos Sociais, Lab Biol Estrutural & Zooquim,Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.sponsorshipNational Institute of Science and Technology on Tuberculosis
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 02/2006
dc.description.sponsorshipIdCNPq: 304051/1975-06
dc.description.sponsorshipIdCNPq: 520182/99-5
dc.description.sponsorshipIdCNPq: 500079/90-0
dc.format.extent2652-2663
dc.identifierhttp://dx.doi.org/10.1099/mic.0.030148-0
dc.identifier.citationMicrobiology-sgm. Reading: Soc General Microbiology, v. 155, p. 2652-2663, 2009.
dc.identifier.doi10.1099/mic.0.030148-0
dc.identifier.issn1350-0872
dc.identifier.lattes2901888624506535
dc.identifier.urihttp://hdl.handle.net/11449/19742
dc.identifier.wosWOS:000269028900019
dc.language.isoeng
dc.publisherSoc General Microbiology
dc.relation.ispartofMicrobiology-sgm
dc.relation.ispartofjcr1.866
dc.relation.ispartofsjr0,924
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleIdentification of Rv3852 as a nucleoid-associated protein in Mycobacterium tuberculosisen
dc.typeArtigo
dcterms.licensehttp://vir.sgmjournals.org/site/misc/ifora.xhtml#req-archiving
dcterms.rightsHolderSoc General Microbiology
unesp.author.lattes2901888624506535
unesp.author.orcid0000-0002-7363-8211[4]
unesp.author.orcid0000-0003-0903-2407[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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Artigos - Biologia - IBRC