Computer-assisted analysis of p53 and PCNA expression in oral lesions infected with human papillomavirus

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Soares, Christiane Pienna
Neto, Carlos Benatti
Fregonezi, Paula Andrea Gabrielli
Teresa, Debora Barreto
Santos, Raimunda Telma de Macedo
Longatto-Filho, Adhemar
Maeda, Marina

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OBJECTIVE: To carry out a retrospective study to determine whether human papillomavirus (HPV) infection and immunohistochemical expression of p53 and proliferating cell nuclear antigen (PCNA) are related to the risk of oral cancer. STUDY DESIGN: Fifty-seven oral biopsies, consisting of 30 oral squamous papillomas (OSPs) and 27 oral squamous cell carcinomas (OSCCs) were tested for the presence of HPV 6/11 and 16/18 by in situ hybridization using catalyzed signal amplification and in situ hybridization. p53 And PCNA expression was analyzed by immunohistochemistry and evaluated quantitatively by image analysis. RESULTS: Nineteen of the 57 oral lesions (33.3%) were positive for HPV. HPV 6/11 was found in 6 of 30 (20%) OSPs and 1 of 27 (3.7%) OSCCs. HPV 16/18 was found in 10 of 27 (37%) OSCCs and 2 of 30 (6.7%) OSPs. Sixteen of the 19 HPV-positive cases (84.2%) were p53 negative; 5 (9%) were HPV 6/11 and 11 (19%) HPV 16/18, with an inverse correlation between the presence of HPV DNA and p53 expression (P=.017, P < .05). PCNA expression appeared in 18 (94.7%) of HPV positive cases, showing that HPV 16/18 was associated with intensity of PCNA expression and with OSCCs (P=.037, P < .05). CONCLUSION: Quantitative evaluation of p53 by image analysis showed an inverse correlation between p53 expression and HPV presence, suggesting protein degradation. Image analysis also demonstrated that PCNA expression was more intense in HPV DNA 16/18 OSCCs. These findings suggest involvement of high-risk HPV types in oral carcinogenesis.



Image analysis, computer-assisted, p53 protein, Papillomavirus, human, Proliferating cell, cycline, protein p53, virus DNA, biopsy, cancer diagnosis, cancer risk, carcinogenesis, catalysis, clinical article, computer analysis, controlled study, correlation analysis, gene amplification, human, human tissue, image analysis, immunohistochemistry, in situ hybridization, mouth cancer, papilloma, priority journal, protein degradation, protein expression, quantitative assay, retrospective study, squamous cell carcinoma, virus infection, Wart virus, Carcinoma, Squamous Cell, Image Processing, Computer-Assisted, Immunoenzyme Techniques, In Situ Hybridization, Mouth Neoplasms, Papilloma, Papillomaviridae, Papillomavirus Infections, Proliferating Cell Nuclear Antigen, Retrospective Studies, Tumor Markers, Biological, Tumor Suppressor Protein p53, Tumor Virus Infections, DNA viruses, Human papillomavirus, Human papillomavirus type 16, Human papillomavirus types, Papillomavirus

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Analytical and Quantitative Cytology and Histology, v. 25, n. 1, p. 19-24, 2003.