Canonical WNT signaling regulates development of bovine embryos to the blastocyst stage

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dc.contributor.authorDenicol, Anna C.
dc.contributor.authorDobbs, Kyle B.
dc.contributor.authorMcLean, Kanyon M.
dc.contributor.authorCarambula, Silvia F.
dc.contributor.authorLoureiro, Barbara [UNESP]
dc.contributor.authorHansen, Peter J.
dc.contributor.institutionUniversity of Florida
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:35Z
dc.date.available2014-05-27T11:28:35Z
dc.date.issued2013-03-01
dc.description.abstractObjectives were to evaluate the role of canonical WNT signaling in development of the preimplantation embryo. Signaling was activated with 2-Amino-4-(3,4-(methylenedioxy)benzylamino)-6-(3-methoxyphenyl)pyrimidine (AMBMP) and inhibited with Dickkopf-related protein 1 (DKK1). Treatment of bovine embryos with AMBMP at day 5 after insemination decreased development to the blastocyst stage at day 7 and reduced numbers of trophectoderm and inner cell mass cells. At high concentrations, AMBMP caused disorganization of the inner cell mass. DKK1 blocked actions of AMBMP but did not affect development in the absence of AMBMP. Examination of gene expression in day 6 morulae by microarray revealed expression of 16 WNT genes and other genes involved in WNT signaling; differences in relative expression were confirmed by PCR for 7 genes. In conclusion, the preimplantation embryo possesses a functional WNT signaling system and activation of the canonical pathway can inhibit embryonic development.en
dc.description.affiliationDepartment of Animal Sciences Genetics Institute University of Florida, Gainesville, FL 32611-0910
dc.description.affiliationDepartamento de Farmacologia Instituto de Biociências Universidade Estadual Paulista (UNESP), Botucatu - SP
dc.description.affiliationUnespDepartamento de Farmacologia Instituto de Biociências Universidade Estadual Paulista (UNESP), Botucatu - SP
dc.identifierhttp://dx.doi.org/10.1038/srep01266
dc.identifier.citationScientific Reports, v. 3.
dc.identifier.doi10.1038/srep01266
dc.identifier.issn2045-2322
dc.identifier.scopus2-s2.0-84874327499
dc.identifier.urihttp://hdl.handle.net/11449/74697
dc.language.isoeng
dc.relation.ispartofScientific Reports
dc.relation.ispartofjcr4.122
dc.relation.ispartofsjr1,533
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subject1,3 benzodioxole derivative
dc.subject2 amino 4 (3,4 (methylenedioxy)benzylamino) 6 (3 methoxyphenyl)pyrimidine
dc.subject2-amino-4-(3,4-(methylenedioxy)benzylamino)-6-(3-methoxyphenyl)pyrimidine
dc.subjectpyrimidine derivative
dc.subjectsignal peptide
dc.subjectWnt protein
dc.subjectanimal
dc.subjectanimal embryo
dc.subjectartificial insemination
dc.subjectblastocyst
dc.subjectcattle
dc.subjectchemistry
dc.subjectcytology
dc.subjectdrug effect
dc.subjectdrug potentiation
dc.subjectembryo development
dc.subjectfemale
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectWnt signaling pathway
dc.subjectAnimals
dc.subjectBenzodioxoles
dc.subjectBlastocyst
dc.subjectCattle
dc.subjectEmbryo, Mammalian
dc.subjectEmbryonic Development
dc.subjectFemale
dc.subjectGene Expression Regulation, Developmental
dc.subjectInsemination
dc.subjectIntercellular Signaling Peptides and Proteins
dc.subjectPyrimidines
dc.subjectWnt Proteins
dc.subjectWnt Signaling Pathway
dc.titleCanonical WNT signaling regulates development of bovine embryos to the blastocyst stageen
dc.typeArtigo
dcterms.licensehttp://www.nature.com/authors/policies/license.html
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Farmacologia - IBB