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Ultra-high-performance liquid chromatography using a fused-core particle column for fast analysis of propolis phenolic compounds

dc.contributor.authorContieri, Letícia S.
dc.contributor.authorde Souza Mesquita, Leonardo M
dc.contributor.authorSanches, Vitor L.
dc.contributor.authorViganó, Juliane
dc.contributor.authorKamikawachi, Renan Canute [UNESP]
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.authorRostagno, Mauricio A.
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T15:42:24Z
dc.date.available2023-07-29T15:42:24Z
dc.date.issued2023-02-01
dc.description.abstractPropolis is a bee product with a complex chemicalcomposition formed by several species from different geographical origins. The complex propolis composition requires an accurate and reproducible characterization of samples to standardize the quality of the material sold to consumers. This work developed an ultra-high-performance liquid chromatography with a photodiode array detector method to analyze propolis phenolic compounds based on the two key propolis biomarkers, Artepillin C and p-Coumaric acid. This choice was made due to the complexity of the sample with the presence of several compounds. The optimized method was hyphenated with mass spectrometry detection allowing the detection of 23 different compounds. A step-by-step strategy was used to optimize temperature, flow rate, mobile phase composition, and re-equilibration time. Reverse-phase separation was achieved with a C18 fused-core column packed with the commercially available smallest particles (1.3 nm). Using a fused-core column with ultra-high-performance liquid chromatography allows highly efficient, sensitive, accurate, and reproducible determination of compounds extracted from propolis with an outstanding sample throughput and resolution. Optimized conditions permitted the separation of the compounds in 5.50 min with a total analysis time (sample-to-sample) of 6.50 min.en
dc.description.affiliationMultidisciplinary Laboratory of Food and Health (LabMAS) School of applied sciences (FCA) University of Campinas (UNICAMP)
dc.description.affiliationCentro de Ciências da Natureza Universidade Federal de São Carlos Rod. Lauri Simões de Barros
dc.description.affiliationUNESP – São Paulo State University Institute of Biosciences
dc.description.affiliationUnespUNESP – São Paulo State University Institute of Biosciences
dc.identifierhttp://dx.doi.org/10.1002/jssc.202200440
dc.identifier.citationJournal of Separation Science, v. 46, n. 3, 2023.
dc.identifier.doi10.1002/jssc.202200440
dc.identifier.issn1615-9314
dc.identifier.issn1615-9306
dc.identifier.scopus2-s2.0-85144013070
dc.identifier.urihttp://hdl.handle.net/11449/249476
dc.language.isoeng
dc.relation.ispartofJournal of Separation Science
dc.sourceScopus
dc.subjectArtepillin C
dc.subjectfast analysis
dc.subjectfused-core
dc.subjectp- Coumaric acid
dc.subjectpropolis
dc.titleUltra-high-performance liquid chromatography using a fused-core particle column for fast analysis of propolis phenolic compoundsen
dc.typeArtigo
unesp.author.orcid0000-0003-1763-5697[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, São Vicentept
unesp.departmentCiências Biológicas - IBCLPpt

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