Comparison of a multiplex-PCR assay with mycolic acids analysis and conventional methods for the identification of Mycobacteria

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dc.contributor.authorTanaka, Ioshie Ibara
dc.contributor.authorAnno, Ivone Shizuko [UNESP]
dc.contributor.authorDe Andrade Leite, Sergio Roberto [UNESP]
dc.contributor.authorCooksey, Robert C.
dc.contributor.authorLeite, Clarice Queico Fujimura [UNESP]
dc.contributor.institutionFaculdade de Medicina de Marília (FAMEMA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCenters for Dis. Controls/Prevention
dc.date.accessioned2014-05-27T11:20:40Z
dc.date.available2014-05-27T11:20:40Z
dc.date.issued2003-06-02
dc.description.abstractA fast, sensitive and cost-effective multiplex-PCR assay for Mycobacterium tuberculosis complex (MTC) and Mycobacterium avium (M. avium) identification for routine diagnosis was evaluated. A total of 158 isolates of mycobacteria from 448 clinical specimens from patients with symptoms of mycobacterial disease were analyzed. By conventional biochemical methods 151 isolates were identified as M. tuberculosis, five as M. avium and two as Mycobacterium chelonae (M. chelonae). Mycolic acid patterns confirmed these results. Multiplex-PCR detected only IS6110 in isolates identified as MTC, and IS1245 was found only in the M. avium isolates. The method applied to isolates from two patients, identified by conventional methods and mycolic acid analysis, one as M. avium and other as M. chelonae, resulted positive for IS6110, suggesting co-infection with M. tuberculosis. These patients were successfully submitted to tuberculosis treatment. The multiplex-PCR method may offer expeditious identification of MTC and M. avium, which may minimize risks for active transmission of these organisms and provide useful treatment information.en
dc.description.affiliationFaculdade de Med. de Marilia, Marília
dc.description.affiliationFaculdade de Ciencias Farmaceuticas UNESP, Araraquara, SP
dc.description.affiliationInstituto de Quimica UNESP, Araraquara, SP
dc.description.affiliationCenters for Dis. Controls/Prevention, Atlanta, GA
dc.description.affiliationDepto. de Ciencias Biologicas Faculdade de Ciencias Farmaceuticas UNESP, Rodovia Araraquara-Jaú km 01, 14801-902 Araraquara, SP
dc.description.affiliationUnespFaculdade de Ciencias Farmaceuticas UNESP, Araraquara, SP
dc.description.affiliationUnespInstituto de Quimica UNESP, Araraquara, SP
dc.description.affiliationUnespDepto. de Ciencias Biologicas Faculdade de Ciencias Farmaceuticas UNESP, Rodovia Araraquara-Jaú km 01, 14801-902 Araraquara, SP
dc.format.extent307-312
dc.identifierhttp://dx.doi.org/10.1111/j.1348-0421.2003.tb03401.x
dc.identifier.citationMicrobiology and Immunology, v. 47, n. 5, p. 307-312, 2003.
dc.identifier.doi10.1111/j.1348-0421.2003.tb03401.x
dc.identifier.issn0385-5600
dc.identifier.lattes2114570774349859
dc.identifier.scopus2-s2.0-0038585025
dc.identifier.urihttp://hdl.handle.net/11449/67317
dc.identifier.wosWOS:000182726900001
dc.language.isoeng
dc.relation.ispartofMicrobiology and Immunology
dc.relation.ispartofjcr1.335
dc.relation.ispartofsjr0,764
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectClassical procedures for identification
dc.subjectMultiplex-PCR
dc.subjectMycobacterium avium
dc.subjectMycobacterium chelonae
dc.subjectMycobacterium tuberculosis
dc.subjectMycolic acids analysis
dc.subjectmycolic acid
dc.subjectbacterial transmission
dc.subjectbacterium identification
dc.subjectcost effectiveness analysis
dc.subjectMycobacterium
dc.subjectMycobacterium chelonei
dc.subjectnonhuman
dc.subjectpolymerase chain reaction
dc.subjectBacterial Typing Techniques
dc.subjectDNA Transposable Elements
dc.subjectDNA, Bacterial
dc.subjectHumans
dc.subjectMycobacterium avium Complex
dc.subjectMycobacterium Infections
dc.subjectMycolic Acids
dc.subjectPolymerase Chain Reaction
dc.subjectSensitivity and Specificity
dc.subjectActinobacteria (class)
dc.subjectBacteria (microorganisms)
dc.subjectCorynebacterineae
dc.subjectuncultured actinomycete
dc.titleComparison of a multiplex-PCR assay with mycolic acids analysis and conventional methods for the identification of Mycobacteriaen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
unesp.author.lattes2114570774349859
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentCiências Biológicas - FCFpt

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Araraquara - FCF - Faculdade de Ciências Farmacêuticas