Immunohistochemistry of factor VIII, histology and morphometry in equine tendon treated with platelet- -rich plasma

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dc.contributor.authorZandim, Bruna Mota
dc.contributor.authorDe Souza, Maria Verônica
dc.contributor.authorFrassy, Luiza Neme
dc.contributor.authorVilória, Marlene Isabel Vargas
dc.contributor.authorMaia, Leandro [UNESP]
dc.contributor.authorFonseca, Cláudio César
dc.contributor.authorValente, Fabrício Luciani
dc.contributor.authorDo Carmo Lopes Moreira, José
dc.contributor.authorMagalhães, Pablo Costa
dc.contributor.institutionUniversidade Federal de Viçosa (UFV)
dc.contributor.institutionClínica Médica de Grandes Animais
dc.contributor.institutionDVT
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionPrevent - Laboratório de Anatomia Patológica e Citopatologia
dc.date.accessioned2022-04-28T18:59:28Z
dc.date.available2022-04-28T18:59:28Z
dc.date.issued2013-01-01
dc.description.abstractThe aim of this research was to verify the therapeutic effects of platelet-rich plasma (PRP) in the treatment of tendinopathy of the superficial digital flexor tendon as assessed by histological and morphometric evaluation, as well as to evaluate early changes in the expression of factor VIII by immunohistochemical analysis during the inflammatory and proliferative phases of healing process of PRP treated tendons compared to placebo. Five days after induction of tendinopathy by collagenase, the forelimbs of six horses were treated with intralesional PRP or 0.9% NaCl. Tendon biopsy was performed at 3 or 16 days after treatments. In both phases and with both treatments, the lesion area was filled with granulation tissue containing scattered collagen fibers with loss of parallelism, fibroblasts with varied morphology, neovascularization and hypertrophied endotenon. These characteristics were more evident during the proliferative phase and no differences were observed between treatments. A negative correlation between collagen fibers and moderately plump fibroblasts was observed. Immunohistochemistry staining was positive for factor VIII, without any differences between the treatments and tendon healing phases.en
dc.description.affiliationMédica-veterinária Programa de Pós-Graduação em Medicina Veterinária Universidade Federal de Viçosa (UFV), Av. PH Rolfs, s/n, Viçosa, MG 36570-000
dc.description.affiliationMédica-veterinária Clínica Médica de Grandes Animais Departamento de Veterinária (DVT), Av. PH Rolfs, s/n, Viçosa, MG 36570-000
dc.description.affiliationMédico-veterinário UFV, Av. PH Rolfs, s/n, Viçosa, MG 36570-000
dc.description.affiliationMédica-veterinária Anatomia Patológica DVT, Av. PH Rolfs, s/n, Viçosa, MG 36570-000
dc.description.affiliationMédico-veterinário Laboratório de Reprodução e Terapia Celular Avançada Universidade Estadual Paulista Júlio de Mesquita Filho, Campus Universitário, Botucatu, SP 18618-000
dc.description.affiliationMédico-veterinário DVT UFV, Av. PH Rolfs, s/n, Viçosa, MG 36570-000
dc.description.affiliationMédico-veterinário DVT Universidade Federal de Viçosa (UFV), Av. PH Rolfs, s/n, Viçosa, MG 36570-000
dc.description.affiliationPrevent - Laboratório de Anatomia Patológica e Citopatologia, Rua Benjamin Araújo, 56, s 110 Centro, Viçosa, MG 36570-000
dc.description.affiliationUnespMédico-veterinário Laboratório de Reprodução e Terapia Celular Avançada Universidade Estadual Paulista Júlio de Mesquita Filho, Campus Universitário, Botucatu, SP 18618-000
dc.format.extent169-184
dc.identifier.citationRevista Brasileira de Medicina Veterinaria, v. 35, n. 2, p. 169-184, 2013.
dc.identifier.issn2527-2179
dc.identifier.issn0100-2430
dc.identifier.scopus2-s2.0-84894383604
dc.identifier.urihttp://hdl.handle.net/11449/220088
dc.language.isopor
dc.relation.ispartofRevista Brasileira de Medicina Veterinaria
dc.sourceScopus
dc.subjectGrowth factors
dc.subjectHorses
dc.subjectPlatelet-rich components
dc.subjectTendinous structures
dc.titleImmunohistochemistry of factor VIII, histology and morphometry in equine tendon treated with platelet- -rich plasmaen
dc.titleImunoistoquímica do fator VIII, histologia e morfometria em tendão de equino tratado com plasma rico em plaquetaspt
dc.typeArtigo

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