Hopanoid Hopene Locates in the Interior of Membranes and Affects Their Properties

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dc.contributor.authorAlvares, Dayane S. [UNESP]
dc.contributor.authorCrosio, Matias
dc.contributor.authorWilke, Natalia
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidad Nacional de Córdoba
dc.date.accessioned2022-04-29T08:35:26Z
dc.date.available2022-04-29T08:35:26Z
dc.date.issued2021-01-01
dc.description.abstractHopanoids are proposed as sterol surrogates in some bacteria, and it has been proved that some hopanoids are able to induce a liquid-order phase state in lipid membranes. The members of this group of molecules have diverse structures, and not all of them have been studied in detail yet. Here, we study membranes with the hopanoid hopene (hop-22 (29)-ene or diploptene), which is the product of the cycling of squalene by squalene-hopene cyclase, and thus is present in the first step of hopanoid biosynthesis. Hopene is particularly interesting because it lacks a polar head group, which opens the question of how does this molecule accommodate in a lipid membrane, and what are the effects promoted by its presence. In order to get an insight into this, we prepared monolayers and bilayers of a phospholipid with hopene and studied their properties in comparison with pure phospholipid membranes, and with the sterol cholesterol or the hopanoid diplopterol. Film stiffness, shear viscosity, and bending dynamics were very affected by the presence of hopene, while zeta-potential, generalized polarization of Laurdan, and conductivity were affected moderately by this molecule. The results suggest that at very low percentages, hopene locates parallel to the phospholipid molecules, while the excess of the hopene molecules stays between leaflets, as previously proposed using molecular dynamics simulations.en
dc.description.affiliationDepartment of Physics UNESP-São Paulo State University Ibilce, São Paulo
dc.description.affiliationFacultad de Ciencias Químicas Departamento de Química Biológica Ranwel Caputto Universidad Nacional de Córdoba
dc.description.affiliationCentro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC) Conicet Universidad Nacional de Córdoba
dc.description.affiliationUnespDepartment of Physics UNESP-São Paulo State University Ibilce, São Paulo
dc.identifierhttp://dx.doi.org/10.1021/acs.langmuir.1c02030
dc.identifier.citationLangmuir.
dc.identifier.doi10.1021/acs.langmuir.1c02030
dc.identifier.issn1520-5827
dc.identifier.issn0743-7463
dc.identifier.scopus2-s2.0-85117156994
dc.identifier.urihttp://hdl.handle.net/11449/229708
dc.language.isoeng
dc.relation.ispartofLangmuir
dc.sourceScopus
dc.titleHopanoid Hopene Locates in the Interior of Membranes and Affects Their Propertiesen
dc.typeArtigo
unesp.author.orcid0000-0002-6521-9148 0000-0002-6521-9148 0000-0002-6521-9148[1]
unesp.author.orcid0000-0002-2342-0193 0000-0002-2342-0193[3]

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