Electrophoretic variants of intracellular catalase of different Candida species

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Data

2004-07-01

Autores

Miyasaka, NRS
Unterkircher, C. S.
Carvalho, P. O.
Shimizu, M. T.

Título da Revista

ISSN da Revista

Título de Volume

Editor

Kluwer Academic Publ

Resumo

Intracellular and extracellular catalases of different species of Candida were investigated using different culture media. All the Candida strains produced intracellular catalase, whose enzymatic activity was detected by non-denaturating polyacrylamide gradient (4-30%) gel electrophoresis. The cell extracts presented a major 230 kDa catalase band and in some strains variants of catalase with different molecular weights were detected. Candida catalase activity was not affected by heating at 50degreesC and incubation with beta-mercaptoethanol, but treatment with sodium dodecyl sulphate inhibited or reduced enzymatic activity. Extracellular enzyme activity was not detected in any of the culture filtrate extracts tested.

Descrição

Palavras-chave

Candida, catalase, electrophoresis, variants, virulence

Como citar

Mycopathologia. Dordrecht: Kluwer Academic Publ, v. 158, n. 2, p. 187-193, 2004.

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