Determination of acetaldehyde in fuel ethanol by high-performance liquid chromatography with electrochemical detection

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Data

2005-04-01

Autores

Saczk, A. A.
Okumura, L. L.
De Oliveira, M. F.
Zanoni, Maria Valnice Boldrin [UNESP]
Stradiotto, Nelson Ramos [UNESP]

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Japan Soc Analytical Chemistry

Resumo

The cyclic voltammetric behavior of acetaldehyde and the derivatized product with 2,4-dinitrophenylhydrazine (DNPHi) has been studied at a glassy carbon electrode. This study was used to optimize the best experimental conditions for its determination by high-performance liquid chromatographic (HPLC) separation coupled with electrochemical detection. The acetaldehyde-2,4-dinitrophenyl.hydrazone (ADNPH) was eluted and separated by a reversed-phase column, C-18, under isocratic conditions with the mobile phase containing a binary mixture of methanol/LiCl(aq) at a concentration of 1.0 x 10(-3) M (80:20 v/v) and a flow rate of 1.0 mL min(-1). The optimum condition for the electrochemical detection of ADNPH was +1.0 V vs. Ag/AgCl as a reference electrode. The proposed method was simple, rapid (analysis time 7 min) and sensitive (detection limit 3.80 mu g L-1) at a signal-to-noise ratio of 3:1. It was also highly selective and reproducible [standard deviation 8.2% +/- 0.36 (n = 5)]. The analytical curve of ADNPH was linear over the range of 3-300 mg L-1 per injection (20 mu L), and the analytical recovery was > 99%.

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Analytical Sciences. Tokyo: Japan Soc Analytical Chemistry, v. 21, n. 4, p. 441-444, 2005.