Multilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distribution

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dc.contributor.authorCaccio, Simone M.
dc.contributor.authorSannella, Anna Rosa
dc.contributor.authorBruno, Antonella
dc.contributor.authorStensvold, Christen R.
dc.contributor.authorDavid, Erica Boarato [UNESP]
dc.contributor.authorGuimaraes, Semiramis [UNESP]
dc.contributor.authorManuali, Elisabetta
dc.contributor.authorMagistrali, Chiara
dc.contributor.authorMahdad, Karim
dc.contributor.authorBeaman, Miles
dc.contributor.authorMaserati, Roberta
dc.contributor.authorTosini, Fabio
dc.contributor.authorPozio, Edoardo
dc.contributor.institutionIst Super Sanita
dc.contributor.institutionIRCCS San Matteo Hosp Fdn
dc.contributor.institutionStatens Serum Inst
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionIst Zooprofilatt Sperimentale Umbria & Marche
dc.contributor.institutionWestern Diagnost Pathol
dc.date.accessioned2018-11-26T15:31:44Z
dc.date.available2018-11-26T15:31:44Z
dc.date.issued2016-11-01
dc.description.abstractThe flagellated protozoan Dientamoeba fragilis is often detected in humans with gastrointestinal symptoms, but it is also commonly found in healthy subjects. As for other intestinal protozoa, the hypothesis that genetically dissimilar parasite isolates differ in their ability to cause symptoms has also been raised for D. fragilis. To date, only two D. fragilis genotypes (1 and 2) have been described, of which genotype 1 largely predominates worldwide. However, very few markers are available for genotyping studies and therefore the extent of genetic variation among isolates remains largely unknown. Here, we performed metagenomics experiments on two D. fragilis-positive stool samples, and identified a number of candidate markers based on sequence similarity to the phylogenetically related species Trichomonas vaginalis. Markers corresponding to structural genes and to genes encoding for proteases were selected for this study, and PCR experiments confirmed their belonging to the D. fragilis genome; two previously described markers (small subunit ribosomal DNA and large subunit of RNA polymerase II) were also included. Using this panel of markers, 111 isolates of human origin were genotyped, all of which, except one, belonged to genotype 1. These isolates had been collected at different times from symptomatic and asymptomatic persons of different age groups in Italy, Denmark, Brazil and Australia. By sequencing approximately 160 kb from 500 PCR products, a very low level of polymorphism was observed across all the investigated loci, suggesting the existence of a major clone of D. fragilis with a widespread geographical distribution. (C) 2016 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.en
dc.description.affiliationIst Super Sanita, Rome, Italy
dc.description.affiliationIRCCS San Matteo Hosp Fdn, Lab Parasitol Microbiol & Virol, Pavia, Italy
dc.description.affiliationStatens Serum Inst, Dept Microbiol & Infect Control, Copenhagen, Denmark
dc.description.affiliationSao Paulo State Univ, Inst Biosci, Dept Parasitol, Sao Paulo, Brazil
dc.description.affiliationIst Zooprofilatt Sperimentale Umbria & Marche, Parasitol Lab, Perugia, Italy
dc.description.affiliationWestern Diagnost Pathol, Myaree, WA, Australia
dc.description.affiliationUnespSao Paulo State Univ, Inst Biosci, Dept Parasitol, Sao Paulo, Brazil
dc.description.sponsorshipItalian Ministry of Health (Ricerca Corrente)
dc.description.sponsorshipDG SANTE of the European Commission
dc.description.sponsorshipIdItalian Ministry of Health (Ricerca Corrente): RC04/12
dc.format.extent793-798
dc.identifierhttp://dx.doi.org/10.1016/j.ijpara.2016.07.002
dc.identifier.citationInternational Journal For Parasitology. Oxford: Elsevier Sci Ltd, v. 46, n. 12, p. 793-798, 2016.
dc.identifier.doi10.1016/j.ijpara.2016.07.002
dc.identifier.fileWOS000387630100004.pdf
dc.identifier.issn0020-7519
dc.identifier.urihttp://hdl.handle.net/11449/159190
dc.identifier.wosWOS:000387630100004
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofInternational Journal For Parasitology
dc.relation.ispartofsjr1,638
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectDientamoeba fragilis
dc.subjectHuman
dc.subjectGenetic markers
dc.subjectMultilocus genotyping
dc.subjectPopulation structure
dc.titleMultilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distributionen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.

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