Canine Subcutaneous Mast Cell Tumors: Cellular Proliferation and KIT Expression as Prognostic Indices

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dc.contributor.authorThompson, J. J.
dc.contributor.authorYager, J. A.
dc.contributor.authorBest, S. J.
dc.contributor.authorPearl, D. L.
dc.contributor.authorCoomber, B. L.
dc.contributor.authorTorres, R. N. [UNESP]
dc.contributor.authorKiupel, M.
dc.contributor.authorFoster, R. A.
dc.contributor.institutionUniversity of Guelph
dc.contributor.institutionYagerBest Histol & Cytol Serv
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionMichigan State University
dc.date.accessioned2014-05-20T13:39:37Z
dc.date.available2014-05-20T13:39:37Z
dc.date.issued2011-01-01
dc.description.abstractMolecular assays are widely used to prognosticate canine cutaneous mast cell tumors (MCT). There is limited information about these prognostic assays used on MCT that arise in the subcutis. The aims of this study were to evaluate the utility of KIT immunohistochemical labeling pattern, c-KIT mutational status (presence of internal tandem duplications in exon 11), and proliferation markers-including mitotic index, Ki67, and argyrophilic nucleolar organizing regions (AgNOR)-as independent prognostic markers for local recurrence and/or metastasis in canine subcutaneous MCT. A case-control design was used to analyze 60 subcutaneous MCT from 60 dogs, consisting of 24 dogs with subsequent local recurrence and 12 dogs with metastasis, as compared to dogs matched by breed, age, and sex with subcutaneous MCT that did not experience these events. Mitotic index, Ki67, the combination of Ki67 and AgNOR, and KIT cellular localization pattern were significantly associated with local recurrence and metastasis, thereby demonstrating their prognostic value for subcutaneous MCT. No internal tandem duplication mutations were detected in exon 11 of c-KIT in any tumors. Because c-KIT mutations have been demonstrated in only 20 to 30% of cutaneous MCT and primarily in tumors of higher grade, the number of subcutaneous MCT analyzed in this study may be insufficient to draw conclusions on the role c-KIT mutations in these tumors.en
dc.description.affiliationUniv Guelph, Ontario Vet Coll, Dept Pathobiol, Guelph, on N1G 2W1, Canada
dc.description.affiliationYagerBest Histol & Cytol Serv, Guelph, ON, Canada
dc.description.affiliationSão Paulo State Univ, UNESP, Sch Vet Med, Dept Vet Clin, Botucatu, SP, Brazil
dc.description.affiliationMichigan State Univ, Coll Vet Med, Comparat Med & Integrat Biol Program, E Lansing, MI 48824 USA
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Sch Vet Med, Dept Vet Clin, Botucatu, SP, Brazil
dc.description.sponsorshipOntario Veterinary College Pet Trust Organization
dc.description.sponsorshipRouse Family Foundation
dc.format.extent169-181
dc.identifierhttp://dx.doi.org/10.1177/0300985810390716
dc.identifier.citationVeterinary Pathology. Thousand Oaks: Sage Publications Inc, v. 48, n. 1, p. 169-181, 2011.
dc.identifier.doi10.1177/0300985810390716
dc.identifier.issn0300-9858
dc.identifier.urihttp://hdl.handle.net/11449/13741
dc.identifier.wosWOS:000287200500014
dc.language.isoeng
dc.publisherSage Publications Inc
dc.relation.ispartofVeterinary Pathology
dc.relation.ispartofjcr1.795
dc.relation.ispartofsjr1,078
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAgNORen
dc.subjectcanineen
dc.subjectcase-control studyen
dc.subjectc-KITen
dc.subjectKi67en
dc.subjectmast cell tumoren
dc.subjectmitotic indexen
dc.subjectprognostic markersen
dc.titleCanine Subcutaneous Mast Cell Tumors: Cellular Proliferation and KIT Expression as Prognostic Indicesen
dc.typeArtigo
dcterms.licensehttp://allenpress.com/
dcterms.rightsHolderSage Publications Inc
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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