Biodegradation of biodiesel/diesel blends by Candida viswanathii

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Soares Junior, Jose [UNESP]
Mariano, Adriano Pinto [UNESP]
de Angelis, Dejanira de Franceschi [UNESP]

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Academic Journals


This work is aimed to assess the aerobic biodegradation of biodiesel/diesel blends (0, 2, 5, 20 and 100%, v/v) by Candida viswanathii. The biodegradation potential of the inoculum was assessed with the redox indicator 2,6-dichlorophenol indophenol (DCPIP) test and with respirometric experiment in biometer flasks (250 mL) used to measure the microbial CO(2) production. In the latter, the inoculum was added to a contaminated soil with the blends (addition of 50 mL of fuel/Kg of soil from a non-contaminated site). C. viswanathii was able to increase significantly (approximately 50% in terms of CO(2) production) the biodegradation in soil of biodiesel/diesel blends and neat biodiesel since it preferable biodegrades biodiesel. Without inoculum the biodegradation of diesel oil was higher than biodiesel and blends (47.3, 51.1, 5.7 and 22.1% in terms of CO(2) production by B2, B5, B20 and B100, respectively) presumably due to the presence of the antioxidant terc-butyl-hydroquinone (TBHQ) in the biodiesel.



Diesel, biodiesel, blends, biodegradation, bioaugmentation, TBHQ

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African Journal of Biotechnology. Victoria Island: Academic Journals, v. 8, n. 12, p. 2774-2778, 2009.