The pre-assembled state of magainin 2 lysine-linked dimer determines its enhanced antimicrobial activity

Lorenzon, Esteban N.; Nobre, Thatyane M.; Caseli, Luciano; Cilli, Eduardo M. [UNESP]; Hora, Gabriel C. A. da; Soares, Thereza A.; Oliveira, Osvaldo N.

The pre-assembled state of magainin 2 lysine-linked dimer determines its enhanced antimicrobial activity

Arquivos

WOS000434747200051.pdf (2.73 MB)

Data

2018-07-01

Autores

Hora, Gabriel C. A. da

Soares, Thereza A.

Oliveira, Osvaldo N.

Editor

Elsevier B.V.

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

Antimicrobial peptides (AMPs) are alternatives to conventional antibiotics against multi-drug resistant bacteria with low potential for developing microbial resistance. The design of such molecules requires understanding of the mechanisms of action, particularly the interaction with bacteria cell membranes. In this work, we determine the mechanism responsible for the higher activity against Escherichia coil of the C-terminal lysine dimer of magainin 2, (MG2)(2)K, in comparison to the monomeric peptide magainin 2 (MG2). Langmuir monolayers and vesicles made with the E. coli lipid extract were used to address the two possible states for the peptide-membrane interaction, namely the binding state and pore state, respectively. The incorporation of MG2 and (MG2)(2)K in lipid monolayers at the air-water interface caused slight differences in surface pressure isotherms and polarization-modulated infrared reflection absorption (PM-IRRAS) spectra, and therefore the difference in activity is not associated with the binding state. In contrast, large differences were observed in the leakage experiments where (MG2)(2)K was shown to disrupt the large unilamellar vesicles to a much higher extent owing to efficient pore formation. The binding and penetration of MG2 and (MG2)(2)K were also probed with molecular dynamics (MD) simulations for bilayers made with 1-palmitoy1-2-oleoyl-sn-g/ycero-3-phosphoethanolamine:1-palmitoy1-2-oleoyl-snglycero-3-phosphoglycerol (POPE:POPG). (MG2)(2)K forms disordered toroidal pores at a significant lower concentration than for MG2. In summary, the combination of experimental and computational simulation results indicated that the pre-assembling state of (MG2)(2)K dimer leads to a reduced number of molecules and shorter time being required to kill E. coli. (C) 2018 Elsevier B.V. All rights reserved.