Canine neutrophils activate effector mechanisms in response to Leishmania infantum

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dc.contributor.authorPereira, Maria
dc.contributor.authorValério-Bolas, Ana
dc.contributor.authorSantos-Mateus, David
dc.contributor.authorAlexandre-Pires, Graça
dc.contributor.authorSantos, Marcos
dc.contributor.authorRodrigues, Armanda
dc.contributor.authorRocha, Hugo
dc.contributor.authorSantos, Ana
dc.contributor.authorMartins, Catarina
dc.contributor.authorTomas, Ana
dc.contributor.authorPassero, Filipe [UNESP]
dc.contributor.authorda Fonseca, Isabel Pereira
dc.contributor.authorSantos-Gomes, Gabriela
dc.contributor.institutionUNL
dc.contributor.institutionEdifício do Trem Auto
dc.contributor.institutionUniversity of Lisbon
dc.contributor.institutionNational Guard
dc.contributor.institutionNew University of Lisbon
dc.contributor.institutionUniversity do Porto
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:15:37Z
dc.date.available2018-12-11T17:15:37Z
dc.date.issued2017-12-15
dc.description.abstractCanine leishmaniosis caused by L. infantum is a severe zoonotic disease. Although macrophages are the definitive host cells, neutrophils are the first cells to encounter the parasite soon after its inoculation in the dermis by the phlebotomine vector. To study the interaction of dog neutrophils and L. infantum promastigotes, blood neutrophils were isolated from healthy donors and the infection was established in vitro. In the majority of the dogs, L. infantum was efficiently phagocytized by neutrophils, and oxidative (superoxide production) and non-oxidative (neutrophil elastase exocytosis) intracellular effector mechanisms were activated, but the release of neutrophil extracellular traps was minimized. Furthermore, promastigotes and culture supernatants induced neutrophil migration, but the prior contact with Leishmania inhibits chemotaxis, which might contribute to neutrophil retention at the inoculation site. Neutrophil-parasite interaction resulted in a decrease in parasite viability, although some intracellular promastigotes survive and maintain their proliferative capacity. These findings indicate that dog neutrophils are competent effector cells able to control the initial L. infantum infection. However, some parasites evade intracellular effector mechanisms and can be transferred to the definitive host cell, the macrophage, contributing to the development of canine leishmaniosis.en
dc.description.affiliationGlobal Health and Tropical Medicine GHMT Instituto de Higiene e Medicina Tropical IHMT Universidade Nova de Lisboa UNL, Rua da Junqueira 100
dc.description.affiliationPolytechnic Institute of Portalegre Higher Agrarian School of Elvas Edifício do Trem Auto, Avenida 14 de Janeiro s/n Apartado 254
dc.description.affiliationInterdisciplinary Animal Health Research Centre Faculty of Veterinary Medicine University of Lisbon, Av. Universidade Técnica
dc.description.affiliationDivision of Veterinary Medicine National Guard, Largo do Carmo
dc.description.affiliationChronic Diseases Research Center Immunology NOVA Medical School New University of Lisbon, Rua Câmara Pestana n° 6, 6-A Edifício CEDOC II
dc.description.affiliationI3S Instituto de Investigação e Inovação em Saúde. IBMC Instituto de Biologia Molecular e Celular and ICBAS Instituto de Ciências Biomédicas Abel Salazar University do Porto, Rua Alfredo Allen, 208
dc.description.affiliationSão Paulo State University (Unesp) Institute of Biosciences São Vicente, Praça Infante Dom Henrique, S/N
dc.description.affiliationUnespSão Paulo State University (Unesp) Institute of Biosciences São Vicente, Praça Infante Dom Henrique, S/N
dc.description.sponsorshipFundação para a Ciência e a Tecnologia
dc.description.sponsorshipIdFundação para a Ciência e a Tecnologia: PTDC/CVT/113121/2009
dc.description.sponsorshipIdFundação para a Ciência e a Tecnologia: PTDC/CVT/118566/2010
dc.description.sponsorshipIdFundação para a Ciência e a Tecnologia: UID/CVT/00276/2013
dc.description.sponsorshipIdFundação para a Ciência e a Tecnologia: UID/Multi/04413/2013
dc.format.extent10-20
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2017.10.008
dc.identifier.citationVeterinary Parasitology, v. 248, p. 10-20.
dc.identifier.doi10.1016/j.vetpar.2017.10.008
dc.identifier.file2-s2.0-85032303512.pdf
dc.identifier.issn1873-2550
dc.identifier.issn0304-4017
dc.identifier.scopus2-s2.0-85032303512
dc.identifier.urihttp://hdl.handle.net/11449/175392
dc.language.isoeng
dc.relation.ispartofVeterinary Parasitology
dc.relation.ispartofsjr1,275
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCanine leishmaniosis
dc.subjectElectron microscopy
dc.subjectNeutrophil elastase
dc.subjectNeutrophil extracellular traps
dc.subjectNeutrophils
dc.subjectSuperoxide
dc.titleCanine neutrophils activate effector mechanisms in response to Leishmania infantumen
dc.typeResenha

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