Immunosensors made with layer-by-layer films on chitosan/gold nanoparticle matrices to detect d-dimer as biomarker for venous thromboembolism

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dc.contributor.authorRodrigues, Valquiria C.
dc.contributor.authorMoraes, Marli L.
dc.contributor.authorSoares, Juliana C.
dc.contributor.authorSoares, Andrey C.
dc.contributor.authorSanfelice, Rafaela
dc.contributor.authorDeffune, Elenice [UNESP]
dc.contributor.authorOliveira, Osvaldo N.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionFederal University of the Triângulo Mineiro
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:21:08Z
dc.date.available2018-12-11T17:21:08Z
dc.date.issued2018-01-01
dc.description.abstractWe report on immunosensors to detect D-dimer, a biomarker of venous thromboembolism, which are made with layer-bylayer (LbL) films containing immobilized anti-D-dimer monoclonal antibody alternated with a layer of chitosan/gold nanoparticles (AuNpChi). Detection was due to irreversible adsorption of the antigen D-dimer on its corresponding antibody according to a Langmuir-Freundlich model, thus giving rise to ellipsoidal structures in scanning electron microscopy images whose size and number increased with D-dimer concentration. The chemical groups involved in the adsorption process were inferred from polarization-modulated infrared reflection absorption (PM-IRRAS) through changes in the amide and carbonyl bands. Detection of D-dimer was made with electrical impedance spectroscopy, electrochemical impedance spectroscopy and cyclic voltammetry. The latter was the most sensitive with a detection limit of 9 × 10-4 μg/mL, sensitivity of 0.27 × 10-6A/μgmL-1 with linear increase from 0 to 1 μg/mL. The selectivity of the immunosensor made with AuNpChi/anti-Ddimer film was verified by noting negligible changes in the cyclic voltammograms when exposed to typical interferents in biological fluids.en
dc.description.affiliationSão Carlos Institute of Physics University of São Paulo
dc.description.affiliationFederal University of São Paulo Unifesp Campus São José Dos Campos
dc.description.affiliationDepartment of Chemical Engineering Federal University of the Triângulo Mineiro
dc.description.affiliationDepartment of Urology Medical School UNESP
dc.description.affiliationUnespDepartment of Urology Medical School UNESP
dc.format.extent891-896
dc.identifierhttp://dx.doi.org/10.1246/bcsj.20180019
dc.identifier.citationBulletin of the Chemical Society of Japan, v. 91, n. 6, p. 891-896, 2018.
dc.identifier.doi10.1246/bcsj.20180019
dc.identifier.issn1348-0634
dc.identifier.issn0009-2673
dc.identifier.scopus2-s2.0-85049117792
dc.identifier.urihttp://hdl.handle.net/11449/176511
dc.language.isoeng
dc.relation.ispartofBulletin of the Chemical Society of Japan
dc.relation.ispartofsjr0,999
dc.relation.ispartofsjr0,999
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectD-dimer
dc.subjectImmunosensor
dc.subjectLayer-by-layer (LbL) film
dc.titleImmunosensors made with layer-by-layer films on chitosan/gold nanoparticle matrices to detect d-dimer as biomarker for venous thromboembolismen
dc.typeArtigo

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