In vitro identification of a stem cell population from canine hair follicle bulge region

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dc.contributor.authorCastro, Raquel V. G. de [UNESP]
dc.contributor.authorTavares, Mariana R. [UNESP]
dc.contributor.authorBressan, Fabiana F.
dc.contributor.authorPieri, Naira C. G.
dc.contributor.authorTrindade Hill, Amanda Baracho [UNESP]
dc.contributor.authorSouza, Aline F.
dc.contributor.authorCruz, Nathan da R. N. [UNESP]
dc.contributor.authorMartins, Daniele S.
dc.contributor.authorAmbrosio, Carlos E.
dc.contributor.authorMeirelles, Flavio V.
dc.contributor.authorGarcia, Joaquim M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-11-26T17:48:38Z
dc.date.available2018-11-26T17:48:38Z
dc.date.issued2018-02-01
dc.description.abstractSkin is an extensive and easily accessible organ possessing various cell types that are constantly renewed. Previous studies have suggested the presence of a stem cell niche at the bulge region of the hair follicle, which contains cells positive for CD200 and CD34. Thus, this study sought to identify these cell populations in canine skin cells using the following methods 1- collecting samples of adult and fetal skin and isolating and culturing these cells using a method of simple enzymatic digestion and 2-testing the cell cultures for CD200 and CD34 in vitro and comparing them with skin tissue samples (in situ). Immunofluorescence results were negative for both CD200 and CD34 in frozen and paraffin embedded tissue, whereas the analysis showed that cultured cells positive for CD34, CD200 and double positive cells could be visualized in different percentages. Additionally, the pluripotency marker OCT4 was positive in the isolated cells. Analysis of CD34, CD200 and OCT4 by RT-qPCR showed that there is expression in fetal and adult cells, although no difference was observed between groups. Our results suggest that bulge stem cells from both fetuses and adult dogs were reported with the use of CD34 and CD200 markers in this study, and further techniques for cell isolation and in vitro cultivation are needed in order to obtain enriched populations of skin stem cells in dogs.en
dc.description.affiliationSao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, Sao Paulo, SP, Brazil
dc.description.affiliationSao Paulo State Univ, Fac Agr & Vet Sci, Dept Vet Clin & Surg, Jaboticabal, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Fac Agr & Vet Sci, Dept Vet Clin & Surg, Jaboticabal, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2015/26818-5
dc.format.extent43-50
dc.identifierhttp://dx.doi.org/10.1016/j.tice.2017.12.003
dc.identifier.citationTissue & Cell. Edinburgh: Churchill Livingstone, v. 50, p. 43-50, 2018.
dc.identifier.doi10.1016/j.tice.2017.12.003
dc.identifier.fileWOS000427074600006.pdf
dc.identifier.issn0040-8166
dc.identifier.urihttp://hdl.handle.net/11449/163977
dc.identifier.wosWOS:000427074600006
dc.language.isoeng
dc.publisherChurchill Livingstone
dc.relation.ispartofTissue & Cell
dc.relation.ispartofsjr0,410
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectSkin
dc.subjectOCT4
dc.subjectCD200
dc.subjectCD34
dc.subjectDog
dc.titleIn vitro identification of a stem cell population from canine hair follicle bulge regionen
dc.typeArtigo
dcterms.rightsHolderChurchill Livingstone
unesp.departmentClínica e Cirurgia Veterinária - FCAVpt
unesp.departmentMedicina Veterinária Preventiva e Reprodução Animal - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias
Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias