Comparison of oxidative stress markers between single and twin gestations in Dorper ewes during pregnancy, delivery and postpartum

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dc.contributor.authorSantarosa, B. P. [UNESP]
dc.contributor.authorDantas, G. N. [UNESP]
dc.contributor.authorFerreira, D. O.L.
dc.contributor.authorHooper, H. B.
dc.contributor.authorSinzato, Y. K. [UNESP]
dc.contributor.authorDamasceno, D. C. [UNESP]
dc.contributor.authorPolizel, D. M.
dc.contributor.authorda Silva, A. A.
dc.contributor.authorGonçalves, R. C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionSecretary of Agriculture and Supply of São Paulo State
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionFederal Rural University of Rio de Janeiro (UFRRJ)
dc.date.accessioned2021-06-25T10:51:08Z
dc.date.available2021-06-25T10:51:08Z
dc.date.issued2021-04-01
dc.description.abstractPregnancy in sheep is a physiological condition characterized by a significant increase in energy demand to ensure adequate foetal growth and development, making them susceptible to oxidative stress. The aim of this study was to analyse the markers of oxidative stress in sheep during pregnancy, parturition and close to peripartum (up to 48 h postpartum), comparing single with twin gestation. Sixty healthy Dorper ewes from 2 to 5 years’ old raised under semi-intensive management were used in this study. Ewes were grouped based on foetal number: Group 1, with single pregnancy (n = 30); Group 2, with a twin pregnancy (n = 30). Blood samples were collected nine times: after artificial insemination (D0, control); at 30 days (D30), 90 days (D90), 120 days (D120), 130 days (D130), and 140 days (D140) of pregnancy; on the day of delivery (DD); at 24 h (PD1) and 48 h (PD2) post-delivery. The concentrations of reduced thiol groups (-SH, P = 0.0155), the enzymatic activities of superoxide dismutase (SOD, P = 0.0009), glutathione peroxidase (GSH-Px, P = 0.0110) and catalase (CAT, P = 0.0001) differed according to fetal number by time. Twin gestation decreased concentration of thiobarbituric acid reactive substances (TBARS; P = 0.0278), and TBARS had differed over time (P < 0.0001), but a fetal number by time interaction was not detected (P = 0.2413). Overall, at peripartum period, there was a higher concentration of SOD and glutathione (-SH and GSH-Px), revealing changes on oxidation and antioxidant activity. TBARS concentration was affected by the number of foetus, considering all experimental periods. In conclusion, as gestation progress, the challenges related to the antioxidant status are more pronounced, especially for twin pregnancy, at the final third of gestation.en
dc.description.affiliationDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP)
dc.description.affiliationSecretary of Agriculture and Supply of São Paulo State
dc.description.affiliationDepartment of Basic Sciences School of Animal Science and Food Engineering (FZEA) University of São Paulo (USP)
dc.description.affiliationLaboratory of Experimental Research on Gynecology and Obstetrics Botucatu Medical School São Paulo State University (UNESP)
dc.description.affiliationDepartment of Animal Science College of Agriculture “Luiz de Queiroz” University of São Paulo, Pádua Dias Avenue, n 11, PO Box 09
dc.description.affiliationDepartment of Veterinary Medicine and Surgery Federal Rural University of Rio de Janeiro (UFRRJ)
dc.description.affiliationUnespDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP)
dc.description.affiliationUnespLaboratory of Experimental Research on Gynecology and Obstetrics Botucatu Medical School São Paulo State University (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.identifierhttp://dx.doi.org/10.1016/j.smallrumres.2021.106333
dc.identifier.citationSmall Ruminant Research, v. 197.
dc.identifier.doi10.1016/j.smallrumres.2021.106333
dc.identifier.issn0921-4488
dc.identifier.scopus2-s2.0-85100386743
dc.identifier.urihttp://hdl.handle.net/11449/207231
dc.language.isoeng
dc.relation.ispartofSmall Ruminant Research
dc.sourceScopus
dc.subjectCatalase
dc.subjectGlutathione peroxidase
dc.subjectReduced thiol groups
dc.subjectSheep
dc.subjectSuperoxide dismutase
dc.subjectThiobarbituric acid reactive substances
dc.titleComparison of oxidative stress markers between single and twin gestations in Dorper ewes during pregnancy, delivery and postpartumen
dc.typeArtigo

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Artigos - Clínica Veterinária - FMVZ
Artigos - Ginecologia e Obstetrícia - FMB