Novel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiae

Página do item simplificado
dc.contributor.authorTaggart, Nathan T. E.
dc.contributor.authorCrabtree, Angela M. A.
dc.contributor.authorCreagh, Jack W. M.
dc.contributor.authorBizarria, Rodolfo M. [UNESP]
dc.contributor.authorLi, Shunji
dc.contributor.authorla Higuera, Ignacio A. de
dc.contributor.authorBarnes, Jonathan E.
dc.contributor.authorShipley, Mason A.
dc.contributor.authorBoyer, Josephine M.
dc.contributor.authorStedman, Kenneth M.
dc.contributor.authorYtreberg, F. Marty
dc.contributor.authorRowley, Paul A.
dc.contributor.institutionUniv Idaho
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionPortland State Univ
dc.date.accessioned2023-07-29T12:01:23Z
dc.date.available2023-07-29T12:01:23Z
dc.date.issued2023-06-01
dc.description.abstractIt has been 49 years since the last discovery of a new virus family in the model yeast Saccharomyces cerevisiae. A large-scale screen to determine the diversity of double-stranded RNA (dsRNA) viruses in S. cerevisiae has identified multiple novel viruses from the family Partitiviridae that have been previously shown to infect plants, fungi, protozoans, and insects. Most S. cerevisiae partitiviruses (ScPVs) are associated with strains of yeasts isolated from coffee and cacao beans. The presence of partitiviruses was confirmed by sequencing the viral dsRNAs and purifying and visualizing isometric, non-enveloped viral particles. ScPVs have a typical bipartite genome encoding an RNA-dependent RNA polymerase (RdRP) and a coat protein (CP). Phylogenetic analysis of ScPVs identified three species of ScPV, which are most closely related to viruses of the genus Cryspovirus from the mammalian pathogenic protozoan Cryptosporidium parvum. Molecular modeling of the ScPV RdRP revealed a conserved tertiary structure and catalytic site organization when compared to the RdRPs of the Picornaviridae. The ScPV CP is the smallest so far identified in the Partitiviridae and has structural homology with the CP of other partitiviruses but likely lacks a protrusion domain that is a conspicuous feature of other partitivirus particles. ScPVs were stably maintained during laboratory growth and were successfully transferred to haploid progeny after sporulation, which provides future opportunities to study partitivirus-host interactions using the powerful genetic tools available for the model organism S. cerevisiae. Author summaryThis article describes the discovery and characterization of multiple strains and species of viruses from the family Partitiviridae in the brewer's and baker's yeast S. cerevisiae. These novel viruses have bipartite genomes packaged in spherical viral particles with structural homology to members of the family Partitiviridae. Strikingly, yeast partitiviruses are most closely related to viruses from human pathogenic protozoa and not partitiviruses of other fungi. As partitiviruses can positively and negatively contribute to a host's physiology (including important human and plant pathogens), the presence of partitiviruses in S. cerevisiae offers a unique opportunity to study the biology of these viruses in a well-developed model system.en
dc.description.affiliationUniv Idaho, Dept Biol Sci, Moscow, ID 83844 USA
dc.description.affiliationSao Paulo State Univ UNESP, Dept Gen & Appl Biol, Rio Claro, SP, Brazil
dc.description.affiliationSao Paulo State Univ UNESP, Ctr Study Social Insects, Rio Claro, SP, Brazil
dc.description.affiliationPortland State Univ, Ctr Life Extreme Environm, Dept Biol, Portland, OR USA
dc.description.affiliationUniv Idaho, Inst Modeling Collaborat & Innovat, Moscow, ID USA
dc.description.affiliationUniv Idaho, Dept Phys, Moscow, ID USA
dc.description.affiliationUnespSao Paulo State Univ UNESP, Dept Gen & Appl Biol, Rio Claro, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ UNESP, Ctr Study Social Insects, Rio Claro, SP, Brazil
dc.description.sponsorshipNational Institute of General Medical Sciences of the National Institutes of Health
dc.description.sponsorshipNational Science Foundation Division of Molecular and Cellular Biosciences
dc.description.sponsorshipNational Science Foundation EPSCoR Research Infrastructure Improvement Program: Track-2
dc.description.sponsorshipNational Institutes of Health
dc.description.sponsorshipOffice of Nuclear Energy of the U.S. DOE
dc.description.sponsorshipNuclear Science User Facilities
dc.description.sponsorshipFunda��o de Amparo � Pesquisa do Estado de S�o Paulo (FAPESP)
dc.description.sponsorshipUniversity of Idaho Office of Undergraduate Research
dc.description.sponsorshipDepartment of Biological Sciences
dc.description.sponsorshipDyess Faculty Fellowship, University of Idaho College of Science
dc.description.sponsorshipIdNational Institute of General Medical Sciences of the National Institutes of Health: P20 GM104420
dc.description.sponsorshipIdNational Science Foundation Division of Molecular and Cellular Biosciences: 1818368
dc.description.sponsorshipIdNational Science Foundation Division of Molecular and Cellular Biosciences: 2025305
dc.description.sponsorshipIdNational Science Foundation EPSCoR Research Infrastructure Improvement Program: Track-2: OIA-1736253
dc.description.sponsorshipIdNational Institutes of Health: P30 GM103324
dc.description.sponsorshipIdNuclear Science User Facilities: DE-AC07-05ID14517
dc.description.sponsorshipIdFAPESP: 2021/09980-4
dc.format.extent22
dc.identifierhttp://dx.doi.org/10.1371/journal.ppat.1011418
dc.identifier.citationPlos Pathogens. San Francisco: Public Library Science, v. 19, n. 6, 22 p., 2023.
dc.identifier.doi10.1371/journal.ppat.1011418
dc.identifier.issn1553-7366
dc.identifier.urihttp://hdl.handle.net/11449/245662
dc.identifier.wosWOS:001003237700002
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos Pathogens
dc.sourceWeb of Science
dc.titleNovel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiaeen
dc.typeArtigo
dcterms.rightsHolderPublic Library Science
unesp.author.orcid0000-0003-1695-4899[5]

Arquivos

Coleções

Artigos - Biologia - IBRC
Rio Claro - CEIS - Centro de Estudos de Insetos Sociais