LC–ESI–IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties

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dc.contributor.authorde Souza Mesquita, Leonardo Mendes [UNESP]
dc.contributor.authorRodrigues, Caroline Fabri Bittencourt [UNESP]
dc.contributor.authorda Rocha, Cláudia Quintino [UNESP]
dc.contributor.authorBianchim, Mayara Silveira [UNESP]
dc.contributor.authorRodrigues, Clenilson Martins
dc.contributor.authorde Oliveira, Vanda Maria
dc.contributor.authorGaeta, Henrique Hessel [UNESP]
dc.contributor.authorBelchor, Mariana Novo [UNESP]
dc.contributor.authorToyama, Marcos Hikari [UNESP]
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUFMA - Federal University of Maranhão
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2019-10-06T16:43:42Z
dc.date.available2019-10-06T16:43:42Z
dc.date.issued2019-02-01
dc.description.abstractAbstract: We recognize the chemical composition of the acetonic extract of Rhizophora mangle barks (AERM) using mass spectrometry analysis [liquid chromatography (LC)–ESI–IT-MS/MS and matrix-assisted laser desorption/ionization-time of flight-MS (MALDI-TOF)]. Analgesic activity was evaluated by formalin and tail-flick experimental assays. Anti-inflammatory activity was performed by paw edema test induced by carrageenan and 48/80 compounds. The first series of experiments involved [LC]–FIA–IT-MS/MS with 11 separated catechins derivatives until degree of polymerization 3 (DP3). The spectra obtained by MALDI-TOF analysis of the AERM presented two homologous series: one based on polymers of m/z 288 Da increments (up to DP12) and another series based on polymers of m/z [288 + 162] Da increments (up to DP11). In addition to these series of flavan-3-ol, each DP had a subset of masses with a variation of − 16 Da (homologous series of afzelechins—m/z 873–3465 Da) and + 16 Da (homologous series of gallocatechins—m/z 905–3497 Da). A similar pattern with homologous series of gallocatechins and afzelechins could also be observed for a fifth and a sixth monohexoside series: glucogallocatechins (m/z 779–3371) and glucoafzelechins (m/z 747–3339). The intraperitoneal administration of different doses of AERM (50, 150 and 300 µg mL−1) have a morphine-like effect and intense anti-inflammatory activity. Graphical Abstract: [Figure not available: see fulltext.].en
dc.description.affiliationLaboratory of Bioprospection of Natural Products (LBPN) UNESP - São Paulo State University/Coastal Campus of São Vicente, Pça Infante Dom Henrique S/N
dc.description.affiliationLaboratório de Estudos Avançados em Fitomedicamentos (LEAF) UFMA - Federal University of Maranhão, Av. dos Portugueses, 1966 - Bacanga
dc.description.affiliationEmbrapa Agroenergy Brazilian Agricultural Research Corporation, W3 Norte, PqEB
dc.description.affiliationUnespLaboratory of Bioprospection of Natural Products (LBPN) UNESP - São Paulo State University/Coastal Campus of São Vicente, Pça Infante Dom Henrique S/N
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2009/52237-9
dc.description.sponsorshipIdFAPESP: 2014/23951-3
dc.format.extent23-34
dc.identifierhttp://dx.doi.org/10.1007/s13659-018-0192-8
dc.identifier.citationNatural Products and Bioprospecting, v. 9, n. 1, p. 23-34, 2019.
dc.identifier.doi10.1007/s13659-018-0192-8
dc.identifier.issn2192-2209
dc.identifier.issn2192-2195
dc.identifier.lattes8573195327542061
dc.identifier.scopus2-s2.0-85070987582
dc.identifier.urihttp://hdl.handle.net/11449/189531
dc.language.isoeng
dc.relation.ispartofNatural Products and Bioprospecting
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAnti-inflammatory
dc.subjectCatechins
dc.subjectMangroves
dc.subjectMorphine-like effect
dc.subjectTannins
dc.titleLC–ESI–IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Propertiesen
dc.typeArtigo
unesp.advisor.lattes8573195327542061

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Artigos - Ciências Biológicas - São Vicente