Evaluation of antimicrobial photodynamic therapy with acidic methylene blue for the treatment of experimental periodontitis

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dc.contributor.authorde Almeida, Juliano Milanezi [UNESP]
dc.contributor.authorMatheus, Henrique Rinaldi [UNESP]
dc.contributor.authorAlves, Breno Edson Sendão [UNESP]
dc.contributor.authorGusman, David Jonathan Rodrigues [UNESP]
dc.contributor.authorNagata, Maria José Hitomi [UNESP]
dc.contributor.authorde Abreu Furquim, Elisa Mara [UNESP]
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-05-01T13:41:33Z
dc.date.available2022-05-01T13:41:33Z
dc.date.issued2022-02-01
dc.description.abstractObjective To investigate the security and effectiveness of antimicrobial photodynamic therapy (aPDT) with a citric acid-based methylene blue (MB) on the periodontal repair following the treatment of ligature-induced experimental periodontitis (EP) in rats. Material and methods Were used 120 male rats, randomly divided into 4 experimental groups (n = 30): no treatment (NT), SRP alone (SRP), SRP plus aPDT using conventional MB pH 7.0 (aPDT-pH7), SRP plus aPDT using acidic MB pH 1.0 (aPDT-pH1). EP was induced at day 0 by the placement of a ligature around the mandibular left first molars. Ten animals per group/period were euthanized at 14, 22 and 37 days. Histopathological, histometric (percentage of bone in the furcation [PBF]) and immunohistochemical (for tartrate-resistant acid phosphatase [TRAP] and osteocalcin [OCN]) analyses were performed. Data were statistically analyzed. Results aPDT-pH1 showed the highest PBF as compared with the other treatments. Collectively, tissues’ reaction to both dyes were controlled and healthy for the periodontium. Both aPDT protocols reduced the extent and intensity of the local inflammatory response, reduced the alveolar bone resorption, and promoted a better structural arrangement of the connective tissue as compared with SRP. TRAP expression was downregulated while OCN expression was upregulated by aPDT as compared with SRP alone. Conclusion Our data implicate that the novel MB pH 1.0 is as safe as the conventional MB for use in aPDT and raises its additional benefit of increasing the amount of alveolar bone in the furcation.en
dc.description.affiliationPeriodontics Division Department of Diagnosis and Surgery School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationSchool of Dentistry Nucleus of Study and Research in Periodontics and Implantology (NEPPI) São Paulo State University (Unesp), SP
dc.description.affiliationDepartment of Basic Science School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationUnespPeriodontics Division Department of Diagnosis and Surgery School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationUnespSchool of Dentistry Nucleus of Study and Research in Periodontics and Implantology (NEPPI) São Paulo State University (Unesp), SP
dc.description.affiliationUnespDepartment of Basic Science School of Dentistry São Paulo State University (Unesp)
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0263103
dc.identifier.citationPLoS ONE, v. 17, n. 2 February, 2022.
dc.identifier.doi10.1371/journal.pone.0263103
dc.identifier.issn1932-6203
dc.identifier.scopus2-s2.0-85124385523
dc.identifier.urihttp://hdl.handle.net/11449/234128
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.sourceScopus
dc.titleEvaluation of antimicrobial photodynamic therapy with acidic methylene blue for the treatment of experimental periodontitisen
dc.typeArtigo

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Artigos - Cirurgia e Clínica Integrada - FOA
Artigos - Ciências Básicas - FOA