Haemonchus contortus: A multiple-resistant Brazilian isolate and the costs for its characterization and maintenance for research use

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dc.contributor.authorChagas, Ana Carolina S.
dc.contributor.authorKatiki, Luciana M.
dc.contributor.authorSilva, Ives C. [UNESP]
dc.contributor.authorGiglioti, Rodrigo [UNESP]
dc.contributor.authorEsteves, Sérgio N.
dc.contributor.authorOliveira, Márcia Cristina S.
dc.contributor.authorBarioni Jr., Waldomiro
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionInstituto de Zootecnia
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:18Z
dc.date.available2014-05-27T11:28:18Z
dc.date.issued2013-02-01
dc.description.abstractThe aim of this work was to determine the resistance level of Haemonchus contortus isolated from the Santa Inês flock of the Embrapa (Brazilian government's Agricultural Research Company), Southeast Livestock Unit (CPPSE), as well as to determine costs of characterizing and maintaining this isolate in host donors. Forty-two male Santa Inês lambs were experimentally infected with 4000 H. contortus infective larvae of the field isolate of CPPSE, called Embrapa2010, and divided into six treatment groups, which received triclorfon, albendazol plus cobalt sulfate, ivermectin, moxidectin, closantel and levamisole phosphate, as well as a negative control group (water). Egg per gram (EPG) counts were performed at 0, 3, 7, 10 and 14. days post treatment when the animals were slaughtered for parasite count. The data were analyzed using the RESO statistical program, considering anthelmintic resistance under 95% of efficacy. EPG and worm count presented a linear and significant relation with 94% determination coefficient. The susceptibility results obtained by RESO through both criteria (EPG and worm count) were equal, except for closantel, showing that the isolate Embrapa2010 is resistant to benzimidazoles, macrocyclic lactones and imidazothiazoles. The need of a control group did not appear to be essential since the result for susceptibility in the analyses with or without this group was the same. Suppression in egg production after treatment did not occur in the ivermectin and moxidectin groups. In the control group, the establishment percentage was just 12.5 because of the low number of third-stage larvae, resistance (innate and infection immunity) of the animals studied plus good nutrition. Drug classes presented similar efficacy between adults and immature stages. The costs for isolate characterization were calculated for 42 animals during 60. days. The total cost based on local market rates was approximately US$ 8000. The precise identification of Brazilian isolates and their establishment in host donors would be useful for laboratorial anthelmintic resistance diagnoses through in vitro tests, which has an annual cost of approximately US$ 2500 for maintenance in host donors. © 2012 Elsevier B.V.en
dc.description.affiliationEmbrapa Pecuária Sudeste, São Carlos, São Paulo
dc.description.affiliationInstituto de Zootecnia, Nova Odessa, São Paulo
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Jaboticabal, São Paulo
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Jaboticabal, São Paulo
dc.format.extent1-6
dc.identifierhttp://dx.doi.org/10.1016/j.parint.2012.07.001
dc.identifier.citationParasitology International, v. 62, n. 1, p. 1-6, 2013.
dc.identifier.doi10.1016/j.parint.2012.07.001
dc.identifier.issn1383-5769
dc.identifier.issn1873-0329
dc.identifier.scopus2-s2.0-84869872289
dc.identifier.urihttp://hdl.handle.net/11449/74478
dc.identifier.wosWOS:000312511100001
dc.language.isoeng
dc.relation.ispartofParasitology International
dc.relation.ispartofjcr2.055
dc.relation.ispartofsjr0,914
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectGastrointestinal nematodes
dc.subjectHost donor
dc.subjectIsolate
dc.subjectResistance
dc.subjectSmall ruminants
dc.subjectalbendazole
dc.subjectbenzimidazole
dc.subjectclosantel
dc.subjectcobalt sulfate
dc.subjectdiantel
dc.subjectivermectin
dc.subjectlevamisole
dc.subjectmacrolide
dc.subjectmetrifonate
dc.subjectmoxidectin
dc.subjectneguvon
dc.subjecttetramisole
dc.subjectunclassified drug
dc.subjectvalbazen10 cobalto
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectBrazil
dc.subjectcontrolled study
dc.subjectcost
dc.subjectdata analysis
dc.subjectdrug resistance
dc.subjectegg production
dc.subjectfeces analysis
dc.subjectfemale
dc.subjecthaemonchosis
dc.subjectHaemonchus contortus
dc.subjectinnate immunity
dc.subjectlamb
dc.subjectlarva
dc.subjectmale
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectworm
dc.subjectAnimals
dc.subjectAnthelmintics
dc.subjectCosts and Cost Analysis
dc.subjectDrug Resistance, Multiple
dc.subjectFemale
dc.subjectHaemonchiasis
dc.subjectHaemonchus
dc.subjectMale
dc.subjectParasite Egg Count
dc.subjectResearch
dc.subjectSheep
dc.subjectSheep Diseases
dc.subjectAnimalia
dc.subjectNematoda
dc.subjectOvis aries
dc.titleHaemonchus contortus: A multiple-resistant Brazilian isolate and the costs for its characterization and maintenance for research useen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.orcid0000-0002-1700-0547[4]
unesp.author.orcid0000-0003-3939-0088[1]
unesp.author.orcid0000-0002-7038-4607[6]

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