Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms

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dc.contributor.authorCastillo Pedraza, Midian C. [UNESP]
dc.contributor.authorNovais, Tatiana F. [UNESP]
dc.contributor.authorFaustoferri, Roberta C.
dc.contributor.authorQuivey, Robert G.
dc.contributor.authorTerekhov, Anton
dc.contributor.authorHamaker, Bruce R.
dc.contributor.authorKlein, Marlise I. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Rochester
dc.contributor.institutionPurdue University
dc.date.accessioned2018-12-11T16:49:45Z
dc.date.available2018-12-11T16:49:45Z
dc.date.issued2017-10-21
dc.description.abstractStreptococcus mutans-derived exopolysaccharides are virulence determinants in the matrix of biofilms that cause caries. Extracellular DNA (eDNA) and lipoteichoic acid (LTA) are found in cariogenic biofilms, but their functions are unclear. Therefore, strains of S. mutans carrying single deletions that would modulate matrix components were used: eDNA–∆lytS and ∆lytT; LTA–∆dltA and ∆dltD; and insoluble exopolysaccharide–ΔgtfB. Single-species (parental strain S. mutans UA159 or individual mutant strains) and mixed-species (UA159 or mutant strain, Actinomyces naeslundii and Streptococcus gordonii) biofilms were evaluated. Distinct amounts of matrix components were detected, depending on the inactivated gene. eDNA was found to be cooperative with exopolysaccharide in early phases, while LTA played a larger role in the later phases of biofilm development. The architecture of mutant strains biofilms was distinct (vs UA159), demonstrating that eDNA and LTA influence exopolysaccharide distribution and microcolony organization. Thus, eDNA and LTA may shape exopolysaccharide structure, affecting strategies for controlling pathogenic biofilms.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationCenter for Oral Biology University of Rochester
dc.description.affiliationWhistler Center for Carbohydrate Research Purdue University
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (Unesp)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipNational Institute of Dental and Craniofacial Research
dc.description.sponsorshipIdFAPESP: 2014/05423-0
dc.description.sponsorshipIdFAPESP: 2014/21355-4
dc.description.sponsorshipIdCNPq: 29716
dc.description.sponsorshipIdCNPq: 35803
dc.description.sponsorshipIdCNPq: 39550
dc.description.sponsorshipIdNational Institute of Dental and Craniofacial Research: DE017425-06
dc.format.extent722-740
dc.identifierhttp://dx.doi.org/10.1080/08927014.2017.1361412
dc.identifier.citationBiofouling, v. 33, n. 9, p. 722-740, 2017.
dc.identifier.doi10.1080/08927014.2017.1361412
dc.identifier.file2-s2.0-85029906153.pdf
dc.identifier.issn1029-2454
dc.identifier.issn0892-7014
dc.identifier.scopus2-s2.0-85029906153
dc.identifier.urihttp://hdl.handle.net/11449/170206
dc.language.isoeng
dc.relation.ispartofBiofouling
dc.relation.ispartofsjr0,835
dc.relation.ispartofsjr0,835
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBiofilms
dc.subjecteDNA
dc.subjectexopolysaccharides
dc.subjectextracellular matrix
dc.subjectlipoteichoic acids
dc.subjectStreptococcus mutans
dc.titleExtracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilmsen
dc.typeArtigo
unesp.author.orcid0000-0001-8204-838X[4]
unesp.author.orcid0000-0002-7916-1557[7]

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