Rutile nano-bio-interactions mediate dissimilar intracellular destiny in human skin cells

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dc.contributor.authorSanches, P. L.
dc.contributor.authorSouza, W.
dc.contributor.authorGemini-Piperni, S.
dc.contributor.authorRossi, A. L.
dc.contributor.authorScapin, S.
dc.contributor.authorMidlej, V
dc.contributor.authorSade, Y.
dc.contributor.authorPaes Leme, A. F.
dc.contributor.authorBenchimol, M.
dc.contributor.authorRocha, L. A. [UNESP]
dc.contributor.authorCarias, R. B.
dc.contributor.authorBorojevic, R.
dc.contributor.authorGranjeiro, J. M.
dc.contributor.authorRibeiro, A. R.
dc.contributor.institutionNatl Inst Metrol Qual & Technol
dc.contributor.institutionUniv Grande Rio
dc.contributor.institutionIBTN
dc.contributor.institutionBrazilian Ctr Res Phys
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionCNPEM
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFASE
dc.contributor.institutionFluminense Fed Univ
dc.date.accessioned2019-10-04T12:39:05Z
dc.date.available2019-10-04T12:39:05Z
dc.date.issued2019-06-01
dc.description.abstractThe use of nanoparticles (NPs) in the healthcare market is growing exponentially, due to their unique physicochemical properties. Titanium dioxide nanoparticles (TiO2 NPs) are used in the formulation of sunscreens, due to their photoprotective capacity, but interactions of these particles with skin cells on the nanoscale are still unexplored. In the present study we aimed to determine whether the initial nano-biological interactions, namely the formation of a nano-bio-complex (other than the protein corona), can predict rutile internalization and intracellular trafficking in primary human fibroblasts and keratinocytes. Results showed no significant effect of NPs on fibroblast and keratinocyte viability, but cell proliferation was possibly compromised due to nano-bio-interactions. The bio-complex formation is dependent upon the chemistry of the biological media and NPs' physicochemical properties, facilitating NP internalization and triggering autophagy in both cell types. For the first time, we observed that the intracellular traffic of NPs is different when comparing the two skin cell models, and we detected NPs within multivesicular bodies (MVBs) of keratinocytes. These structures grant selected input of molecules involved in the biogenesis of exosomes, responsible for cell communication and, potentially, structural equilibrium in human tissues. Nanoparticle-mediated alterations of exosome quality, quantity and function can be another major source of nanotoxicity.en
dc.description.affiliationNatl Inst Metrol Qual & Technol, Directory Life Sci Appl Metrol, Av Nossa Senhora das Gracas 50, Xerem, RJ, Brazil
dc.description.affiliationUniv Grande Rio, Postgrad Program Translat Biomed, Rua Prof Jose de Souza Herdy 1160, Duque De Caxias, Brazil
dc.description.affiliationIBTN, Brazilian Branch, Rio De Janeiro, Brazil
dc.description.affiliationNatl Inst Metrol Qual & Technol, Postgrad Program Biotechnol, Av Nossa Senhora das Gracas 50, Xerem, RJ, Brazil
dc.description.affiliationBrazilian Ctr Res Phys, R Dr Xavier Sigaud 150, Rio De Janeiro, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Av Brg Trompowski, Rio De Janeiro, Brazil
dc.description.affiliationCNPEM, Lab Nacl Biociencias, R Giuseppe Maximo Scalfara 10000, Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Phys Dept, Av Engn Luiz Edmundo Carrijo Coube, Bauru, SP, Brazil
dc.description.affiliationFASE, Fac Med, Ctr Regenerat Med, Av Barao do Rio Branco 1003, Petropolis, RJ, Brazil
dc.description.affiliationFluminense Fed Univ, Dent Sch, R Miguel de Frias 9, Niteroi, RJ, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Phys Dept, Av Engn Luiz Edmundo Carrijo Coube, Bauru, SP, Brazil
dc.description.sponsorshipINMETRO
dc.description.sponsorshipFINEP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipJovem Cientista do Nosso Estado fellowship
dc.description.sponsorshipPROPESQUnigranrio/Funadesp fellowship
dc.description.sponsorshipIdFAPERJ: 2009045272
dc.description.sponsorshipIdFAPERJ: 111.313/2014APQ1 2014/01
dc.description.sponsorshipIdFAPERJ: E-26/111.313/2014
dc.description.sponsorshipIdCNPq: 490761/2013-5
dc.description.sponsorshipIdCAPES: 99999.008666/2014-08
dc.format.extent2216-2228
dc.identifierhttp://dx.doi.org/10.1039/c9na00078j
dc.identifier.citationNanoscale Advances. Cambridge: Royal Soc Chemistry, v. 1, n. 6, p. 2216-2228, 2019.
dc.identifier.doi10.1039/c9na00078j
dc.identifier.issn2516-0230
dc.identifier.urihttp://hdl.handle.net/11449/185840
dc.identifier.wosWOS:000472766100011
dc.language.isoeng
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofNanoscale Advances
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleRutile nano-bio-interactions mediate dissimilar intracellular destiny in human skin cellsen
dc.typeArtigo
dcterms.rightsHolderRoyal Soc Chemistry
unesp.departmentFísica - FCpt

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