Presence of autoantibodies against HeLa small nuclear ribonucleoproteins in chagasic and non-chagasic cardiac patients

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dc.contributor.authorBosetto, M.C. [UNESP]
dc.contributor.authorPeixoto, M.S. [UNESP]
dc.contributor.authorCastro, L.I.R. de [UNESP]
dc.contributor.authorCicarelli, R.M.B. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:25:24Z
dc.date.available2014-05-20T13:25:24Z
dc.date.issued2004-01-01
dc.description.abstractWe detected anti-human small nuclear ribonucleoprotein (snRNP) autoantibodies in chagasic patients by different immunological methods using HeLa snRNPs. ELISA with Trypanosoma cruzi total lysate antigen or HeLa human U small nuclear ribonucleoproteins (UsnRNPs) followed by incubation with sera from chronic chagasic and non-chagasic cardiac patients was used to screen and compare serum reactivity. Western blot analysis using a T. cruzi total cell extract was also performed in order to select some sera for Western blot and immunoprecipitation assays with HeLa nuclear extract. ELISA showed that 73 and 95% of chronic chagasic sera reacted with HeLa UsnRNPs and T. cruzi antigens, respectively. The Western blot assay demonstrated that non-chagasic cardiac sera reacted with high molecular weight proteins present in T. cruzi total extract, probably explaining the 31% reactivity found by ELISA. However, these sera reacted weakly with HeLa UsnRNPs, in contrast to the chagasic sera, which showed autoantibodies with human Sm (from Stefanie Smith, the first patient in whom this activity was identified) proteins (B/B', D1, D2, D3, E, F, and G UsnRNP). Immunoprecipitation reactions using HeLa nuclear extracts confirmed the reactivity of chagasic sera and human UsnRNA/RNPs, while the other sera reacted weakly only with U1snRNP. These findings agree with previously reported data, thus supporting the idea of the presence of autoimmune antibodies in chagasic patients. Interestingly, non-chagasic cardiac sera also showed reactivity with T. cruzi antigen and HeLa UsnRNPs, which suggests that individuals with heart disease of unknown etiology may develop autoimmune antibodies at any time. The detection of UsnRNP autoantibodies in chagasic patients might contribute to our understanding of how they develop upon initial T. cruzi infection.en
dc.description.affiliationUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências Biológicas
dc.description.affiliationUnespUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências Biológicas
dc.format.extent37-45
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2004000100006
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 1, p. 37-45, 2004.
dc.identifier.doi10.1590/S0100-879X2004000100006
dc.identifier.fileS0100-879X2004000100006.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2004000100006
dc.identifier.urihttp://hdl.handle.net/11449/8038
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica (ABRADIC)
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.relation.ispartofjcr1.492
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectChagas' diseaseen
dc.subjectAutoantibodiesen
dc.subjectSmall nuclear ribonucleoproteinen
dc.subjectTrypanosoma cruzien
dc.subjectHeLa cellsen
dc.subjectCross-reactivityen
dc.titlePresence of autoantibodies against HeLa small nuclear ribonucleoproteins in chagasic and non-chagasic cardiac patientsen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentCiências Biológicas - FCFpt

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Araraquara - FCF - Faculdade de Ciências Farmacêuticas