Antimicrobial Resistance and Virulence Factors of Escherichia coli in Cheese Made from Unpasteurized Milk in Three Cities in Brazil

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dc.contributor.authorRibeiro, Laryssa Freitas [UNESP]
dc.contributor.authorBarbosa, Mayhara Martins Cordeiro
dc.contributor.authorPinto, Fernanda de Rezende
dc.contributor.authorMaluta, Renato Pariz
dc.contributor.authorOliveira, Monica Costa [UNESP]
dc.contributor.authorSouza, Viviane de
dc.contributor.authorMedeiros, Maria Izabel Merino de
dc.contributor.authorBorges, Lucimara Antonio [UNESP]
dc.contributor.authorAmaral, Luiz Augusto do [UNESP]
dc.contributor.authorFairbrother, John Morris
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Fed Educ Ciencia & Tecnol Ceara IFCE
dc.contributor.institutionUniv Fed Pelotas
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionInst Tecnol Alimentos
dc.contributor.institutionUniv Montreal
dc.date.accessioned2018-11-26T17:06:14Z
dc.date.available2018-11-26T17:06:14Z
dc.date.issued2016-09-01
dc.description.abstractThe production of cheeses from unpasteurized milk is still widespread in Brazil, even with a legal ban imposed on its marketing. The manufacture of this cheese is a public health problem, due to the use of raw milk and the poor hygienic conditions throughout the supply chain process. Contamination may occur from several sources and involve several different pathogenic microorganisms, such as Escherichia coli. The latter can cause different clinical manifestations depending on the pathotype involved. Furthermore, some isolates manifest antimicrobial resistance and may be a risk for public health. The purpose of the current study was to investigate the presence of potentially pathogenic E. coli in raw-milk cheese in Brazil and their possible risk to public health. A total of 83 cheeses were collected from three different cities and 169 E. coli isolates were characterized for the presence of enteropathogenic E. coli, Shigatoxigenic E. coli, enterotoxigenic E. coli, extraintestinal pathogenic E. coli (ExPEC) virulence genes, phylogenetic type, antimicrobial resistance, O serogroup, and pulsed-field gel electrophoresis. The number of samples positive for E. coli was highest in Aracaju (90.32%, 28/31). The prevalence of samples positive for potential ExPEC genes was similar for Uberaba and Aracaju (23.07%); the most prevalent ExPEC virulence genes were tsh, iucD, and papC. Isolates from Uberaba had a higher prevalence of resistance to tetracycline (38.46%), amoxicillin/clavulanic acid (58.85%), and ampicillin (61.54%) than the other cities. Overall, antimicrobial resistance genes tetB, bla(TEM), and bla(CMY-2) were the most prevalent genes (26.32%, 15.79%, and 28.95%, respectively) and the most prevalent serotypes were O4 (8%), 018 (12%), and O23 (8%). Clones originating from the same regions and from different regions were observed. These results emphasize the presence of a potential danger for humans in the consumption of raw-milk cheeses in three cities in Brazil due to the presence of antimicrobial resistance, which should be monitored.en
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, Brazil
dc.description.affiliationInst Fed Educ Ciencia & Tecnol Ceara IFCE, Quixada, Brazil
dc.description.affiliationUniv Fed Pelotas, Dept Vet Prevent, Pelotas, Brazil
dc.description.affiliationUniv Estadual Campinas UNICAMP, Inst Biol, Dept Genet Evolucao & Bioagentes, Campinas, SP, Brazil
dc.description.affiliationEmbrapa Caprinos & Ovinos, Sobral, Brazil
dc.description.affiliationInst Tecnol Alimentos, Bauru, Brazil
dc.description.affiliationUniv Montreal, Fac Med Vet, Dept Pathol & Microbiol, 3200 Rue Sicotte, St Hyacinthe, PQ J2S 2M2, Canada
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2011/04451-1
dc.format.extent469-476
dc.identifierhttp://dx.doi.org/10.1089/fpd.2015.2106
dc.identifier.citationFoodborne Pathogens And Disease. New Rochelle: Mary Ann Liebert, Inc, v. 13, n. 9, p. 469-476, 2016.
dc.identifier.doi10.1089/fpd.2015.2106
dc.identifier.issn1535-3141
dc.identifier.urihttp://hdl.handle.net/11449/161935
dc.identifier.wosWOS:000383582100001
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc
dc.relation.ispartofFoodborne Pathogens And Disease
dc.relation.ispartofsjr1,063
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleAntimicrobial Resistance and Virulence Factors of Escherichia coli in Cheese Made from Unpasteurized Milk in Three Cities in Brazilen
dc.typeArtigo
dcterms.rightsHolderMary Ann Liebert, Inc
unesp.departmentMedicina Veterinária Preventiva e Reprodução Animal - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias