Assessment of apoptosis in epidermal lamellar cells in clinically normal horses and those with laminitis

Página do item simplificado
dc.contributor.authorFaleiros, R. R.
dc.contributor.authorStokes, A. M.
dc.contributor.authorEades, S. C.
dc.contributor.authorKim, D. Y.
dc.contributor.authorPaulsen, D. B.
dc.contributor.authorMoore, R. M.
dc.contributor.institutionLouisiana State Univ
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:22:46Z
dc.date.available2014-05-20T15:22:46Z
dc.date.issued2004-05-01
dc.description.abstractObjective - To determine and compare the number, type, location, and distribution of apoptotic epidermal cells in the laminae of clinically normal horses and horses with laminitis.Sample Population - Formalin-fixed samples of digital lamellar tissue from 47 horses (including clinically normal horses [controls; n = 7], horses with acurte [4] and chronic [7] naturally acquired laminitis, and horses with black walnut extract-induced [11] or carbohydrate overload-induced [18] laminitis).Procedure - Blocks of paraffin-embedded lamellar tissues were stained for DNA fragmentation with the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) technique. Differential immunohistochemical staining for caspases 3 and 14 were used to confirm apoptosis.Results - the number of TUNEL-positive epidermal cells per 0.1 mm of primary laminae was significantly greater in the acute laminitis group than in the other groups. In the acute laminitis group, there were 17 and 1,025 times as many TUN EL-positive basal layer cells and keratinocytes, respectively, compared with the control group. Apoptosis of TUNEL-positive basal layer cells was confirmed by results of caspase 3 immunohistochemical staining. The TUNEL-positive keratinocytes did not stain for caspases 3 or 14.Conclusions and Clinical Relevance - the large number of apoptotic basal layer cells detected in the lamellar tissue of horses with acute naturally acquired laminitis suggests that apoptosis may be important in the development of acute laminitis. The role of the large number of TUNEL-positive keratinocytes detected in the interface of primary and secondary epidermal laminae of horses with acute laminitis remains to be elucidated.en
dc.description.affiliationLouisiana State Univ, Sch Vet Med, Dept Vet Clin Sci, Equine Hlth Studies Program, Baton Rouge, LA 70803 USA
dc.description.affiliationLouisiana State Univ, Sch Vet Med, Dept Pathobiol Sci, Baton Rouge, LA 70803 USA
dc.description.affiliationUniv Estadual Paulista, Fac Ciências Agrarias & Vet, Post Grad Program Vet Surg, Jaboticabal, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências Agrarias & Vet, Post Grad Program Vet Surg, Jaboticabal, SP, Brazil
dc.format.extent578-585
dc.identifierhttp://dx.doi.org/10.2460/ajvr.2004.65.578
dc.identifier.citationAmerican Journal of Veterinary Research. Schaumburg: Amer Veterinary Medical Assoc, v. 65, n. 5, p. 578-585, 2004.
dc.identifier.doi10.2460/ajvr.2004.65.578
dc.identifier.issn0002-9645
dc.identifier.urihttp://hdl.handle.net/11449/33696
dc.identifier.wosWOS:000221002000007
dc.language.isoeng
dc.publisherAmer Veterinary Medical Assoc
dc.relation.ispartofAmerican Journal of Veterinary Research
dc.relation.ispartofjcr0.833
dc.relation.ispartofsjr0,567
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleAssessment of apoptosis in epidermal lamellar cells in clinically normal horses and those with laminitisen
dc.typeArtigo
dcterms.licensehttps://www.avma.org/News/Journals/Pages/journals-reprints.aspx
dcterms.rightsHolderAmer Veterinary Medical Assoc
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Clínica e Cirurgia Veterinária - FCAV