Insights into temperature modulation of the Eucalyptus globulus and Eucalyptus grandis antioxidant and lignification subproteomes

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dc.contributor.authorSantana Costa, Marilia Gabriela de [UNESP]
dc.contributor.authorMazzafera, Paulo
dc.contributor.authorBalbuena, Tiago Santana [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2018-11-26T17:28:19Z
dc.date.available2018-11-26T17:28:19Z
dc.date.issued2017-05-01
dc.description.abstractEucalyptus grandis and Eucalyptus globulus are among the most widely cultivated trees, differing in lignin composition and plantation areas, as E. grandis is mostly cultivated in tropical regions while E. globulus is preferred in temperate areas. As temperature is a key modulator in plant metabolism, a large-scale proteome analysis was carried out to investigate changes in the antioxidant system and the lignificadon metabolism in plantlets grown at different temperatures. Our strategy allowed the identification of 3111 stem proteins. A total of 103 antioxidant proteins were detected in the stems of both species. Hierarchical clustering revealed that alterations in the antioxidant proteins are more prominent when Eucalyptus seedlings were exposed to high temperature and that the superoxide isoforms coded by the gene Eucgr.B03930 are the most abundant antioxidant enzymes induced by thermal stimulus. Regarding the lignin biosynthesis, our proteomics approach resulted in the identification of 13 of the 17 core proteins involved in this metabolism, corroborating with gene predictions and the proposed lignin toolbox. Quantitative analyses revealed significant differences in 8 protein isoforms, including the ferulate 5-hydroxylase isoform F5H1, a key enzyme in catalyzing the synthesis of sinapyl alcohol, and the cinnamyl alcohol dehydrogenase isoform CAD2, the last enzyme in monolignol biosynthesis. Data are available via ProteomeXchange with identifier PXD005743. (C) 2017 Elsevier Ltd. All rights reserved.en
dc.description.affiliationSao Paulo State Univ, Dept Technol, Fac Agr & Vet Sci, Jaboticabal, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Dept Plant Biol, Campinas, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Dept Technol, Fac Agr & Vet Sci, Jaboticabal, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 2011/11650-0
dc.description.sponsorshipIdFAPESP: 2014/23541-0
dc.format.extent15-23
dc.identifierhttp://dx.doi.org/10.1016/j.phytochem.2017.01.017
dc.identifier.citationPhytochemistry. Oxford: Pergamon-elsevier Science Ltd, v. 137, p. 15-23, 2017.
dc.identifier.doi10.1016/j.phytochem.2017.01.017
dc.identifier.fileWOS000399508300002.pdf
dc.identifier.issn0031-9422
dc.identifier.urihttp://hdl.handle.net/11449/162721
dc.identifier.wosWOS:000399508300002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPhytochemistry
dc.relation.ispartofsjr1,048
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectLignocellulose biomass
dc.subjectMass spectrometry
dc.subjectPlant-environment interaction
dc.subjectTree physiology
dc.titleInsights into temperature modulation of the Eucalyptus globulus and Eucalyptus grandis antioxidant and lignification subproteomesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.departmentTecnologia - FCAVpt

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