Insights into temperature modulation of the Eucalyptus globulus and Eucalyptus grandis antioxidant and lignification subproteomes
dc.contributor.author | Santana Costa, Marilia Gabriela de [UNESP] | |
dc.contributor.author | Mazzafera, Paulo | |
dc.contributor.author | Balbuena, Tiago Santana [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Universidade Estadual de Campinas (UNICAMP) | |
dc.date.accessioned | 2018-11-26T17:28:19Z | |
dc.date.available | 2018-11-26T17:28:19Z | |
dc.date.issued | 2017-05-01 | |
dc.description.abstract | Eucalyptus grandis and Eucalyptus globulus are among the most widely cultivated trees, differing in lignin composition and plantation areas, as E. grandis is mostly cultivated in tropical regions while E. globulus is preferred in temperate areas. As temperature is a key modulator in plant metabolism, a large-scale proteome analysis was carried out to investigate changes in the antioxidant system and the lignificadon metabolism in plantlets grown at different temperatures. Our strategy allowed the identification of 3111 stem proteins. A total of 103 antioxidant proteins were detected in the stems of both species. Hierarchical clustering revealed that alterations in the antioxidant proteins are more prominent when Eucalyptus seedlings were exposed to high temperature and that the superoxide isoforms coded by the gene Eucgr.B03930 are the most abundant antioxidant enzymes induced by thermal stimulus. Regarding the lignin biosynthesis, our proteomics approach resulted in the identification of 13 of the 17 core proteins involved in this metabolism, corroborating with gene predictions and the proposed lignin toolbox. Quantitative analyses revealed significant differences in 8 protein isoforms, including the ferulate 5-hydroxylase isoform F5H1, a key enzyme in catalyzing the synthesis of sinapyl alcohol, and the cinnamyl alcohol dehydrogenase isoform CAD2, the last enzyme in monolignol biosynthesis. Data are available via ProteomeXchange with identifier PXD005743. (C) 2017 Elsevier Ltd. All rights reserved. | en |
dc.description.affiliation | Sao Paulo State Univ, Dept Technol, Fac Agr & Vet Sci, Jaboticabal, SP, Brazil | |
dc.description.affiliation | Univ Estadual Campinas, Inst Biol, Dept Plant Biol, Campinas, SP, Brazil | |
dc.description.affiliationUnesp | Sao Paulo State Univ, Dept Technol, Fac Agr & Vet Sci, Jaboticabal, SP, Brazil | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description.sponsorshipId | FAPESP: 2011/11650-0 | |
dc.description.sponsorshipId | FAPESP: 2014/23541-0 | |
dc.format.extent | 15-23 | |
dc.identifier | http://dx.doi.org/10.1016/j.phytochem.2017.01.017 | |
dc.identifier.citation | Phytochemistry. Oxford: Pergamon-elsevier Science Ltd, v. 137, p. 15-23, 2017. | |
dc.identifier.doi | 10.1016/j.phytochem.2017.01.017 | |
dc.identifier.file | WOS000399508300002.pdf | |
dc.identifier.issn | 0031-9422 | |
dc.identifier.uri | http://hdl.handle.net/11449/162721 | |
dc.identifier.wos | WOS:000399508300002 | |
dc.language.iso | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation.ispartof | Phytochemistry | |
dc.relation.ispartofsjr | 1,048 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Web of Science | |
dc.subject | Lignocellulose biomass | |
dc.subject | Mass spectrometry | |
dc.subject | Plant-environment interaction | |
dc.subject | Tree physiology | |
dc.title | Insights into temperature modulation of the Eucalyptus globulus and Eucalyptus grandis antioxidant and lignification subproteomes | en |
dc.type | Artigo | |
dcterms.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
dcterms.rightsHolder | Elsevier B.V. | |
unesp.department | Tecnologia - FCAV | pt |
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