Coupling Surface-Enhanced Resonance Raman Scattering and Electronic Tongue as Characterization Tools to Investigate Biological Membrane Mimetic Systems
dc.contributor.author | Aoki, Pedro H. B. [UNESP] | |
dc.contributor.author | Alessio, Priscila [UNESP] | |
dc.contributor.author | Riul, Antonio | |
dc.contributor.author | De Saja Saez, J. A. | |
dc.contributor.author | Constantino, Carlos J. L. [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Univ Valladolid | |
dc.contributor.institution | Universidade Federal de São Carlos (UFSCar) | |
dc.date.accessioned | 2014-05-20T13:23:06Z | |
dc.date.available | 2014-05-20T13:23:06Z | |
dc.date.issued | 2010-05-01 | |
dc.description.abstract | The surface-enhanced Raman scattering (SERS) effect and sensor and biosensor analyses are widely applied to investigate drug-biomolecule interactions or to detect trace amount of analytes. In this work, surface-enhanced resonance Raman scattering (SERRS) and an electronic tongue system using impedance spectroscopy were brought together, combining sensitivity and structural level information. Taking advantage of the use of layer-by-layer (LbL) films of phospholipids as biological membrane mimetic systems, cardiolipin (CLP) and dipalmitoyl phosphatidyl glycerol (DPPG) were applied as transducers onto Pt interdigitated electrodes forming an array of sensing units. This e-tongue system was able to detect the phenothiazine methylene blue (MB) below nanomolar concentrations. SERRS was applied to investigate the MB molecular arrangement (monomers or aggregates) when in contact with the phospholipids at trace levels of concentration. The key point was the adsorption of Ag nanoparticles (AgNPs) within the phospholipid LbL films. This approach did not compromise the e-tongue performance and allowed the recording of in situ SERRS spectra for the LbL films after immersion into MB aqueous solutions. The detection of MB through SERRS gave similar results to those reported in the literature but now with an unprecedented sensitivity. | en |
dc.description.affiliation | Unesp Univ Estadual Paulista, Fac Ciencias & Tecnol, BR-19060900 Presidente Prudente, SP, Brazil | |
dc.description.affiliation | Univ Valladolid, Fac Sci, E-47011 Valladolid, Spain | |
dc.description.affiliation | Universidade Federal de São Carlos (UFSCar), BR-18043970 Campus Sorocaba, SP, Brazil | |
dc.description.affiliationUnesp | Unesp Univ Estadual Paulista, Fac Ciencias & Tecnol, BR-19060900 Presidente Prudente, SP, Brazil | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description.sponsorship | MICINN from Spain | |
dc.description.sponsorshipId | CAPES: 118/06 | |
dc.description.sponsorshipId | MICINN from Spain: PHB2005-0057-PC | |
dc.format.extent | 3537-3546 | |
dc.identifier | http://dx.doi.org/10.1021/ac902585a | |
dc.identifier.citation | Analytical Chemistry. Washington: Amer Chemical Soc, v. 82, n. 9, p. 3537-3546, 2010. | |
dc.identifier.doi | 10.1021/ac902585a | |
dc.identifier.issn | 0003-2700 | |
dc.identifier.lattes | 7384168674539702 | |
dc.identifier.lattes | 9727122203219263 | |
dc.identifier.orcid | 0000-0003-4701-6408 | |
dc.identifier.orcid | 0000-0002-1345-0540 | |
dc.identifier.uri | http://hdl.handle.net/11449/6911 | |
dc.identifier.wos | WOS:000277213400025 | |
dc.language.iso | eng | |
dc.publisher | Amer Chemical Soc | |
dc.relation.ispartof | Analytical Chemistry | |
dc.relation.ispartofjcr | 6.042 | |
dc.relation.ispartofsjr | 2,362 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.title | Coupling Surface-Enhanced Resonance Raman Scattering and Electronic Tongue as Characterization Tools to Investigate Biological Membrane Mimetic Systems | en |
dc.type | Artigo | |
dcterms.license | http://pubs.acs.org/page/4authors/jpa/index.html | |
dcterms.rightsHolder | Amer Chemical Soc | |
unesp.author.lattes | 7384168674539702[1] | |
unesp.author.lattes | 9727122203219263[2] | |
unesp.author.lattes | 6118325967319836[5] | |
unesp.author.orcid | 0000-0002-1345-0540[2] | |
unesp.author.orcid | 0000-0002-9760-1851[3] | |
unesp.author.orcid | 0000-0003-4701-6408[1] | |
unesp.author.orcid | 0000-0002-5921-3161[5] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Ciências e Tecnologia, Presidente Prudente | pt |
unesp.department | Física, Química e Biologia - FCT | pt |
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