Coupling Surface-Enhanced Resonance Raman Scattering and Electronic Tongue as Characterization Tools to Investigate Biological Membrane Mimetic Systems

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dc.contributor.authorAoki, Pedro H. B. [UNESP]
dc.contributor.authorAlessio, Priscila [UNESP]
dc.contributor.authorRiul, Antonio
dc.contributor.authorDe Saja Saez, J. A.
dc.contributor.authorConstantino, Carlos J. L. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Valladolid
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2014-05-20T13:23:06Z
dc.date.available2014-05-20T13:23:06Z
dc.date.issued2010-05-01
dc.description.abstractThe surface-enhanced Raman scattering (SERS) effect and sensor and biosensor analyses are widely applied to investigate drug-biomolecule interactions or to detect trace amount of analytes. In this work, surface-enhanced resonance Raman scattering (SERRS) and an electronic tongue system using impedance spectroscopy were brought together, combining sensitivity and structural level information. Taking advantage of the use of layer-by-layer (LbL) films of phospholipids as biological membrane mimetic systems, cardiolipin (CLP) and dipalmitoyl phosphatidyl glycerol (DPPG) were applied as transducers onto Pt interdigitated electrodes forming an array of sensing units. This e-tongue system was able to detect the phenothiazine methylene blue (MB) below nanomolar concentrations. SERRS was applied to investigate the MB molecular arrangement (monomers or aggregates) when in contact with the phospholipids at trace levels of concentration. The key point was the adsorption of Ag nanoparticles (AgNPs) within the phospholipid LbL films. This approach did not compromise the e-tongue performance and allowed the recording of in situ SERRS spectra for the LbL films after immersion into MB aqueous solutions. The detection of MB through SERRS gave similar results to those reported in the literature but now with an unprecedented sensitivity.en
dc.description.affiliationUnesp Univ Estadual Paulista, Fac Ciencias & Tecnol, BR-19060900 Presidente Prudente, SP, Brazil
dc.description.affiliationUniv Valladolid, Fac Sci, E-47011 Valladolid, Spain
dc.description.affiliationUniversidade Federal de São Carlos (UFSCar), BR-18043970 Campus Sorocaba, SP, Brazil
dc.description.affiliationUnespUnesp Univ Estadual Paulista, Fac Ciencias & Tecnol, BR-19060900 Presidente Prudente, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipMICINN from Spain
dc.description.sponsorshipIdCAPES: 118/06
dc.description.sponsorshipIdMICINN from Spain: PHB2005-0057-PC
dc.format.extent3537-3546
dc.identifierhttp://dx.doi.org/10.1021/ac902585a
dc.identifier.citationAnalytical Chemistry. Washington: Amer Chemical Soc, v. 82, n. 9, p. 3537-3546, 2010.
dc.identifier.doi10.1021/ac902585a
dc.identifier.issn0003-2700
dc.identifier.lattes7384168674539702
dc.identifier.lattes9727122203219263
dc.identifier.orcid0000-0003-4701-6408
dc.identifier.orcid0000-0002-1345-0540
dc.identifier.urihttp://hdl.handle.net/11449/6911
dc.identifier.wosWOS:000277213400025
dc.language.isoeng
dc.publisherAmer Chemical Soc
dc.relation.ispartofAnalytical Chemistry
dc.relation.ispartofjcr6.042
dc.relation.ispartofsjr2,362
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleCoupling Surface-Enhanced Resonance Raman Scattering and Electronic Tongue as Characterization Tools to Investigate Biological Membrane Mimetic Systemsen
dc.typeArtigo
dcterms.licensehttp://pubs.acs.org/page/4authors/jpa/index.html
dcterms.rightsHolderAmer Chemical Soc
unesp.author.lattes7384168674539702[1]
unesp.author.lattes9727122203219263[2]
unesp.author.lattes6118325967319836[5]
unesp.author.orcid0000-0002-1345-0540[2]
unesp.author.orcid0000-0002-9760-1851[3]
unesp.author.orcid0000-0003-4701-6408[1]
unesp.author.orcid0000-0002-5921-3161[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Tecnologia, Presidente Prudentept
unesp.departmentFísica, Química e Biologia - FCTpt

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Presidente Prudente - FCT - Faculdade de Ciências e Tecnologia