REEVALUATION OF CHLORIDES REGULATION OF HEMOGLOBIN OXYGEN-UPTAKE - THE NEGLECTED CONTRIBUTION OF PROTEIN HYDRATION IN ALLOSTERISM

dc.contributor.authorColombo, M. F.
dc.contributor.authorRau, D. C.
dc.contributor.authorParsegian, V. A.
dc.contributor.institutionNIH
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:27:50Z
dc.date.available2014-05-20T15:27:50Z
dc.date.issued1994-10-25
dc.description.abstractWe have measured hemoglobin oxygen uptake vs. The partial pressure of oxygen, with independently controlled activities of chloride and water. This control is effected by combining different concentrations of NaCl and sucrose in the bathing solution to achieve: (i) water activities were varied and CI- activity was fixed, (ii) both water and CI- activities were varied with a traditional NaCI titration, or (iii) CI- activities were varied and water activity was fixed by adding compensating sucrose. Within this analysis, the CI--regulated loading of four oxygens can be described by the reaction Hb.CI- + 4 O-2 + 65 H2O reversible arrow Hb.4O(2).65H(2)O + CI-. The dissociation of a neatly integral chloride, rather than the nonintegral 1.6 chlorides inferred earlier from simple salt titration, demonstrates the need to recognize the potentially large contribution from changes in water activity when titrating weakly binding solutes. The single-chloride result might simplify structural considerations of the action of CI- in hemoglobin regulation.en
dc.description.affiliationNIH,DIV COMP RES & TECHNOL,STRUCT BIOL LAB,BETHESDA,MD 20892
dc.description.affiliationUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,INST BIOCIENCIAS LETRAS & CIENCIAS EXACTAS,DEPT FIS,BR-15054000 S JOSE RIO PR,SP,BRAZIL
dc.description.affiliationUnespUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,INST BIOCIENCIAS LETRAS & CIENCIAS EXACTAS,DEPT FIS,BR-15054000 S JOSE RIO PR,SP,BRAZIL
dc.format.extent10517-10520
dc.identifierhttp://dx.doi.org/10.1073/pnas.91.22.10517
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America. Washington: Natl Acad Sciences, v. 91, n. 22, p. 10517-10520, 1994.
dc.identifier.doi10.1073/pnas.91.22.10517
dc.identifier.issn0027-8424
dc.identifier.lattes3425817209646054
dc.identifier.urihttp://hdl.handle.net/11449/37751
dc.identifier.wosWOS:A1994PP69800058
dc.language.isoeng
dc.publisherNatl Acad Sciences
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America
dc.relation.ispartofjcr9.504
dc.relation.ispartofsjr6,092
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleREEVALUATION OF CHLORIDES REGULATION OF HEMOGLOBIN OXYGEN-UPTAKE - THE NEGLECTED CONTRIBUTION OF PROTEIN HYDRATION IN ALLOSTERISMen
dc.typeArtigo
dcterms.licensehttp://www.pnas.org/site/misc/authorlicense.pdf
dcterms.rightsHolderNatl Acad Sciences
unesp.author.lattes3425817209646054
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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