Effect of Extracts, Fractions, and Isolated Molecules of Casearia sylvestris to Control Streptococcus mutans Cariogenic Biofilm

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dc.contributor.authorRibeiro, Sabrina M. [UNESP]
dc.contributor.authorBueno, Paula C. P. [UNESP]
dc.contributor.authorCavalheiro, Alberto José [UNESP]
dc.contributor.authorKlein, Marlise I. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionFederal University of Alfenas
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2023-07-29T13:43:36Z
dc.date.available2023-07-29T13:43:36Z
dc.date.issued2023-02-01
dc.description.abstractThe effects of extracts, fractions, and molecules of Casearia sylvestris to control the cariogenic biofilm of Streptococcus mutans were evaluated. First, the antimicrobial and antibiofilm (initial and pre-formed biofilms) in prolonged exposure (24 h) models were investigated. Second, formulations (with and without fluoride) were assessed for topical effects (brief exposure) on biofilms. Third, selected treatments were evaluated via bacterium growth inhibition curves associated with gene expression and scanning electron microscopy. In initial biofilms, the ethyl acetate (AcOEt) and ethanolic (EtOH) fractions from Brasília (BRA/DF; 250 µg/mL) and Presidente Venceslau/SP (Water/EtOH 60:40 and Water/EtOH 40:60; 500 µg/mL) reduced ≥6-logs vs. vehicle. Only the molecule Caseargrewiin F (CsF; 125 µg/mL) reduced the viable cell count of pre-formed biofilms (5 logs vs. vehicle). For topical effects, no formulation affected biofilm components. For the growth inhibition assay, CsF yielded a constant recovery of surviving cells (≅3.5 logs) until 24 h (i.e., bacteriostatic), and AcOEt_BRA/DF caused progressive cell death, without cells at 24 h (i.e., bactericidal). CsF and AcOEt_BRA/DF damaged S. mutans cells and influenced the expression of virulence genes. Thus, an effect against biofilms occurred after prolonged exposure due to the bacteriostatic and/or bactericidal capacity of a fraction and a molecule from C. sylvestris.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), SP
dc.description.affiliationDepartment of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP), SP
dc.description.affiliationDepartment of Physics and Chemistry Faculty of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo (USP), SP
dc.description.affiliationDepartment of Organic Chemistry Institute of Chemistry Federal University of Alfenas, MG
dc.description.affiliationDepartment of Oral Diagnosis Piracicaba Dental School State University of Campinas (UNICAMP), SP
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), SP
dc.description.affiliationUnespDepartment of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP), SP
dc.identifierhttp://dx.doi.org/10.3390/antibiotics12020329
dc.identifier.citationAntibiotics, v. 12, n. 2, 2023.
dc.identifier.doi10.3390/antibiotics12020329
dc.identifier.issn2079-6382
dc.identifier.scopus2-s2.0-85148892072
dc.identifier.urihttp://hdl.handle.net/11449/248420
dc.language.isoeng
dc.relation.ispartofAntibiotics
dc.sourceScopus
dc.subjectbiofilm
dc.subjectCasearia sylvestris
dc.subjectdental caries
dc.subjectextracellular matrix
dc.subjectStreptococcus mutans
dc.titleEffect of Extracts, Fractions, and Isolated Molecules of Casearia sylvestris to Control Streptococcus mutans Cariogenic Biofilmen
dc.typeArtigo
unesp.author.orcid0000-0002-7916-1557[4]

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