A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases


A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.



Nitrile hydratase, Amidase, Nitrilase, Amidase inhibitor, High-throughput screening

Como citar

Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 46, n. 1, p. 113-116, 2015.