Seed cryopreservation, in vitro propagation and ex vitro growth of Cattleya walkeriana Gardner, a vulnerable ornamental orchid

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dc.contributor.authorGaldiano, Renato Fernandes [UNESP]
dc.contributor.authorVendrame, Wagner Aparecido
dc.contributor.authorMoretto, Cristiane [UNESP]
dc.contributor.authorde Faria, Ricardo Tadeu
dc.contributor.authorLemos, Eliana Gertrudes de Macedo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionTropical Research and Education Center
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.date.accessioned2022-04-29T08:03:54Z
dc.date.available2022-04-29T08:03:54Z
dc.date.issued2017-04-01
dc.description.abstractThe aim of this investigation was to evaluate cryopreservation by vitrification of seeds from the orchid species Cattleya walkeriana and to achieve ex vitro acclimatization of in vitro derived cryopreserved seedlings. Seeds were removed from eight-month-old and ten-month-old capsules, and immersed in LN under different PVS2 exposure times. In in vitro experiment, ten different treatments and five replications with approximately 600 seeds each one were distributed in a completely random design and germination was calculated by the number of germinated seeds developing into protocorms and subsequently into seedlings. Seeds that were immersed directly into LN (0 min PVS2) and germinated in 1/2 MS culture medium after plunged directly into LN, returned germination rates of 3.0 (eight-month-old) and 36.8% (ten-month-old). Ten-month-old seeds in vitrification for 1h in PVS2 returned 53% germination in 1/2 MS culture medium. However, when the same mature seeds were exposed for 1h to PVS2 with the addition of 1% phloroglucinol, germination was 4.36% higher compared to the treatment without this additive. Seedlings from cryopreserved seeds showed normal development after 210 days of in vitro culture. Seedlings from ten-month-old seeds cryopreserved for 1h in PVS2 were acclimatized in greenhouse conditions in either sphagnum moss or sphagnum moss with pine bark (1:1, v/v) revealed the best ex vitro growth. Seed cryopreservation of this orchid species has shown to be efficient, demonstrating the effect of seed maturation time and ex vitro seedling acclimatization was established, with successful development of seedlings.en
dc.description.affiliationDepartment of Technology Univ Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato Castellane, s/n
dc.description.affiliationUniversity of Florida Tropical Research and Education Center, 18905 SW 280th St
dc.description.affiliationUniversidade Estadual de Londrina Centro de Ciências Agrárias, Caixa Postal 6001
dc.description.affiliationUnespDepartment of Technology Univ Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato Castellane, s/n
dc.format.extent485-490
dc.identifierhttp://dx.doi.org/10.21475/ajcs.17.11.04.384
dc.identifier.citationAustralian Journal of Crop Science, v. 11, n. 4, p. 485-490, 2017.
dc.identifier.doi10.21475/ajcs.17.11.04.384
dc.identifier.issn1835-2707
dc.identifier.issn1835-2693
dc.identifier.scopus2-s2.0-85018353910
dc.identifier.urihttp://hdl.handle.net/11449/228310
dc.language.isoeng
dc.relation.ispartofAustralian Journal of Crop Science
dc.sourceScopus
dc.subjectAcclimatization
dc.subjectGermplasm conservation
dc.subjectOrchidaceae
dc.subjectPhloroglucinol
dc.subjectVitrification
dc.titleSeed cryopreservation, in vitro propagation and ex vitro growth of Cattleya walkeriana Gardner, a vulnerable ornamental orchiden
dc.typeArtigo
unesp.departmentTecnologia - FCAVpt

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