Susceptibility of Candida albicans, Staphylococcus aureus, and Streptococcus mutans biofilms to photodynamic inactivation: an in vitro study

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dc.contributor.authorPereira, Cristiane Aparecida [UNESP]
dc.contributor.authorRomeiro, Rogerio Lima [UNESP]
dc.contributor.authorBorges Pereira Costa, Anna Carolina [UNESP]
dc.contributor.authorSilva Machado, Ana Karina [UNESP]
dc.contributor.authorJunqueira, Juliana Campos [UNESP]
dc.contributor.authorCardoso Jorge, Antonio Olavo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:04:29Z
dc.date.available2014-05-20T14:04:29Z
dc.date.issued2011-05-01
dc.description.abstractThe purpose of this study was to evaluate specific effects of photodynamic inactivation (PDI) using methylene blue as photosensitizer and low-power laser irradiation on the viability of single-, dual-, and three-species biofilms formed by C. albicans, S. aureus, and S. mutans. Biofilms were grown in acrylic discs immersed in sterile brain heart infusion broth (BHI) containing 5% sucrose, inoculated with microbial suspension (10(6) cells/ml) and incubated for 5 days. on the fifth day, the effects of the methylene blue (MB) photosensitizer at a concentration of 0.1 mg/ml for 5 min and InGaAlP laser (660 nm) for 98 s, alone and conjugated were evaluated. Next, the discs were placed in tubes with sterile physiological solution [0.9% sodium chloride (NaCl)] and sonicated for to disperse the biofilms. Ten-fold serial dilutions were carried and aliquots seeded in selective agar, which were then incubated for 48 h. Then the numbers CFU/ml (log(10)) were counted and analyzed statistically (ANOVA, Tukey test, p < 0.05). Scanning electron microscopy (SEM) on discs treated with PDI and control biofilms groups was performed. Significant decreases in the viability of all microorganisms were observed for biofilms exposed to PDI mediated by MB dye. Reductions (log(10)) of single-species biofilms were greater (2.32-3.29) than the association of biofilms (1.00-2.44). Scanning electron microscopy micrographs suggested that lethal photosensitization occurred predominantly in the outermost layers of the biofilms. The results showed that PDI mediated by MB dye, might be a useful approach for the control of oral biofilms.en
dc.description.affiliationUNESP Univ Estadual Paulista, Sch Dent Sao Jose Campos, Dept Biosci & Oral Diag, BR-12245000 Sao Dimas, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Sch Dent Sao Jose Campos, Dept Biosci & Oral Diag, BR-12245000 Sao Dimas, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 09/52048-1
dc.description.sponsorshipIdFAPESP: 10/00879-4
dc.description.sponsorshipIdFAPESP: 09/12005-1
dc.format.extent341-348
dc.identifierhttp://dx.doi.org/10.1007/s10103-010-0852-3
dc.identifier.citationLasers In Medical Science. London: Springer London Ltd, v. 26, n. 3, p. 341-348, 2011.
dc.identifier.doi10.1007/s10103-010-0852-3
dc.identifier.issn0268-8921
dc.identifier.lattes0322020541055900
dc.identifier.lattes0053567153623569
dc.identifier.urihttp://hdl.handle.net/11449/22627
dc.identifier.wosWOS:000289109800010
dc.language.isoeng
dc.publisherSpringer London Ltd
dc.relation.ispartofLasers in Medical Science
dc.relation.ispartofjcr1.949
dc.relation.ispartofsjr0,713
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBiofilmen
dc.subjectCandida albicansen
dc.subjectStaphylococcus aureusen
dc.subjectStreptococcus mutansen
dc.subjectPhotodynamic inactivationen
dc.titleSusceptibility of Candida albicans, Staphylococcus aureus, and Streptococcus mutans biofilms to photodynamic inactivation: an in vitro studyen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer London Ltd
unesp.author.lattes0322020541055900
unesp.author.lattes0053567153623569[6]
unesp.author.orcid0000-0002-1747-6158[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, São José dos Campospt

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