Reduced insulin secretion in response to nutrients in islets from malnourished young rats is associated with a diminished calcium uptake

dc.contributor.authorLatorraca, M. Q.
dc.contributor.authorCarneiro, E. M.
dc.contributor.authorMello, MAR
dc.contributor.authorBoschero, A. C.
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniv Fed Mato Grosso
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:20:21Z
dc.date.available2014-05-20T15:20:21Z
dc.date.issued1999-01-01
dc.description.abstractChanges in Ca-45 uptake and insulin secretion in response to glucose, leucine, and arginine were measured in isolated islets derived from 4-week-old rats born of mothers maintained with normal protein (NP, 17%) or low protein (LP, 6%) diet during pregnancy and lactation. Glucose provoked a dose-dependent stimulation of insulin secretion in both groups of islets, with basal (2.8 mmol/L glucose) and maximal release (27.7 mmol/L glucose) significantly reduced in LP compared with NP islets. In the LP group the concentration-response curve to glucose was shifted to the right compared with the NP group, with the half-maximal response occurring at 16.9 and 13.3 mmol/L glucose, respectively. In LP islets, glucose-induced first and second phases of insulin secretions were drastically reduced. In addition, insulin response to individual amino acids, or in association with glucose, was also significantly reduced in the LP group compared with NP islets. Finally, in LP islets the Ca-45 uptake after 5 minutes or 90 minutes of incubation (which reflect mainly the entry and retention, respectively, of Ca2+), was lower than in NP islets. These data indicate that in malnourished rats both initial and sustained phases of insulin secretion in response to glucose were reduced. This poor secretory response to nutrients seems to be the consequence of an altered Ca2+ handling by malnourished islet cells. (J. Nutr. Biochem. 10:37-43, 1999) (C) Elsevier B.V. 1999. All rights reserved.en
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Dept Fisiol & Biofis, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Fed Mato Grosso, Fac Enfermagem & Nutr, Dept Nutr & Dietet, Ciuaba, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Educ Fis, Rio Claro, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Educ Fis, Rio Claro, Brazil
dc.format.extent37-43
dc.identifierhttp://dx.doi.org/10.1016/S0955-2863(98)00080-1
dc.identifier.citationJournal of Nutritional Biochemistry. New York: Elsevier B.V., v. 10, n. 1, p. 37-43, 1999.
dc.identifier.doi10.1016/S0955-2863(98)00080-1
dc.identifier.issn0955-2863
dc.identifier.urihttp://hdl.handle.net/11449/31672
dc.identifier.wosWOS:000078079300006
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Nutritional Biochemistry
dc.relation.ispartofjcr4.414
dc.relation.ispartofsjr1,678
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleReduced insulin secretion in response to nutrients in islets from malnourished young rats is associated with a diminished calcium uptakeen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0003-3829-8570[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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